| Usefulness of epifluorescence for quantitative analysis of lactobacilli in probiotic feed. | |
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MedLine Citation:
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PMID: 11851819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: Enumeration of total, active or viable probiotic micro-organisms from liquid or solid commercial feedstuffs was studied during processing and storage. METHODS AND RESULTS: After sample preparation, an epifluorescence microscopy technique and a plating method were investigated comparatively. It was shown that (i) on the day of manufacture, active or viable bacteria were in equivalent amounts and that viable numbers then decreased, depending on the different processing and storage factors enhancing ABNC production, (ii) the amount of total and active lactobacilli remained close and quite stable for months at a high level (>10(8) active fluorescent units). CONCLUSIONS: Processing and storage promoted ABNC cells in the products tested. Consequently, both techniques should be used to evaluate the viable-dead-active status of bacteria for which functional properties are claimed. SIGNIFICANCE AND IMPACT OF THE STUDY: Enumeration of the whole probiotic bacterial population should be take into account for guidelines and labelling since non-viable bacteria could have a probiotic effect. |
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Authors:
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M Bernardeau; J P Vernoux; M Gueguen |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied microbiology Volume: 91 ISSN: 1364-5072 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2001 Dec |
Date Detail:
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Created Date: 2002-02-19 Completed Date: 2002-05-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1103-9 Citation Subset: IM |
Affiliation:
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Sorbial S.A.S., Le Mans Cedex, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques Colony Count, Microbial Food Handling / methods Food Microbiology* Food Preservation Lactobacillus / growth & development, isolation & purification* Microscopy, Fluorescence Probiotics* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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