Document Detail

Usefulness of antibodies for evaluating the biological significance of AGEs.
MedLine Citation:
PMID:  18079488     Owner:  NLM     Status:  MEDLINE    
Polyclonal and monoclonal antibodies have been widely applied to demonstrate the presence of advanced glycation end products (AGEs) in vivo. However, our previous study showed that monoclonal anti-AGE antibody (6D12) and polyclonal anti-N epsilon-(carboxymethyl)lysine (CML) antibody recognize not only CML but also N epsilon-(carboxyethyl)lysine (CEL), thus indicating that we should pay attention to the specificity of the antibodies. As a result, we prepared specific monoclonal antibodies against CML, CEL, N omega-(carboxymethyl)arginine (CMA), and S-(carboxymethyl)cysteine (CMC). Our immunochemical study using anti-CMA antibody demonstrated that the CMA content increased in a time-dependent manner when collagen was incubated with glucose, indicating that immunological quantification using the specific antibody is especially useful for measuring an acid-labile AGE structure, such as CMA. Monoclonal antibody is also applied to identify a novel biological marker in pathological lesions. We prepared antibody libraries against proteins modified with aldehydes, such as glyoxal, methylglyoxal, and glycolaldehyde (GA), and one antibody, GA5, which specifically reacts with the GA-modified protein that is recognized in human atherosclerotic lesions. Following successive high-performance liquid chromatography purification, the GA5-reactive compound was isolated and its chemical structure was found to be 3-hydroxy-4-hydroxymethyl-1-(5-amino-5-carboxypentyl) pyridinium cation, which was named GA-pyridine. Taken together, these results demonstrate that a specific antibody is a powerful tool for analyzing novel biomarkers, formation pathways, and the efficacy of AGE inhibitors.
Ryoji Nagai; Yukio Fujiwara; Katsumi Mera; Keita Motomura; Yasunori Iwao; Keiichiro Tsurushima; Mime Nagai; Kazuhiro Takeo; Makiko Yoshitomi; Masaki Otagiri; Tsuyoshi Ikeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-13
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1126     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-01     Completed Date:  2008-08-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  38-41     Citation Subset:  IM    
Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
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MeSH Terms
Aldehydes / analysis,  immunology
Arginine / analogs & derivatives,  analysis
Glycosylation End Products, Advanced / analysis*,  immunology
Lysine / analogs & derivatives,  analysis
Reg. No./Substance:
0/Aldehydes; 0/Antibodies; 0/Glycosylation End Products, Advanced; 0/N(omega)-carboxymethylarginine; 56-87-1/Lysine; 5746-04-3/N(6)-carboxymethyllysine; 74-79-3/Arginine

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