| Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors. | |
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MedLine Citation:
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PMID: 16782649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: The aim of this work is to investigate the usefulness of a modified protocol of the SOS Chromotest to detect antigenotoxicity activities against gamma-rays of plant extracts with proven antioxidant activity, and to elucidate the antigenotoxic mechanisms involved in radioprotection using this system. MATERIALS AND METHODS: The methodology developed was assayed with amifostine, the most studied radioprotector, and with Phyllanthus orbicularis HBK, Cymbopogon citratus (DC) Stapf and Pinus caribaea Morelet extracts, using pre- and post-treatment procedures. RESULTS: The P. caribaea and C. citratus extracts were antigenotoxic against gamma-rays when the cells were pre-treated with both extracts, suggesting a possible antigenotoxic action through a free radical scavenging mechanisms. Amifostine and the P. orbicularis extract were also antigenotoxic under pre- and post-treatment conditions, indicating that several antimutagenic components of this plant extract may also operate by some intracellular mechanism, unlike its antioxidant activity. CONCLUSIONS: The results have demonstrated the usefulness of the modified SOS Chromotest assay in the screening of phytochemical radioprotectors as well as in the study of their antimutagenic mechanisms. |
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Authors:
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Jorge Luis Fuentes; Alena Alonso; Elizabeth Cuétara; Mariolys Vernhe; Nadine Alvarez; Angel Sánchez-Lamar; Montserrat Llagostera |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of radiation biology Volume: 82 ISSN: 0955-3002 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-06-19 Completed Date: 2006-08-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: England |
Other Details:
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Languages: eng Pagination: 323-9 Citation Subset: IM; S |
Affiliation:
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Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Habana. jlfuentes2005@yahoo.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Bacterial
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genetics,
radiation effects Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Drug Evaluation, Preclinical Escherichia coli / drug effects*, genetics, radiation effects* Mutagenicity Tests / methods* Phytotherapy / methods Plant Extracts / administration & dosage* Plants, Medicinal / chemistry* Radiation Dosage Radiation-Protective Agents / administration & dosage SOS Response (Genetics) / drug effects*, radiation effects* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Plant Extracts; 0/Radiation-Protective Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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