Document Detail

Use of ultrasonic pachymetry for measurement of changes in corneal thickness in mouse corneal transplant rejection.
MedLine Citation:
PMID:  19822919     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Diagnosis of rejection in the mouse model of corneal transplantation is based on subjective judgement of loss of graft transparency. The aims of this study were to (1) evaluate a pachymetry technique to measure changes in mouse corneal thickness and (2) correlate increases in transplant thickness with clinical and histological features of rejection. METHODS: Orthotopic corneal allografts (C57BL/6 strain donor) and syngeneic grafts were performed in A/J mice. Graft transparency was graded and corneal thickness measured by pachymetry on alternate days. Transverse sections of donor cornea excised from eyes representative of clinical opacity grades 1-4 were prepared, photographed, graft section thickness measured and stromal graft-infiltrating cells counted. Intraobserver and interobserver variations in pachymetry were statistically tested. RESULTS: Graft thickness, as measured by pachymetry, increased with each clinical opacity grade. Thickness for opacity grades 0, 1 and 2 was less than 300 microm in all recipients. Graft thickness for grades 3 and 4 was greater than 300 microm in all cases. For measurements up to 400 mum, there was a good correlation between thickness as measured by in vivo pachymetry and in histopathological sections. The mean interobserver bias was -11.35 microm, while the mean intraobserver bias was +3.96 microm. Stromal cellularity increased with increasing corneal thickness up to approximately 300 microm. CONCLUSION: In vivo graft pachymetry provides a new and reliable way to objectively diagnose rejection in the mouse model of corneal transplantation.
T H Flynn; M Ohbayashi; M Dawson; M Siddique; S J Ono; D F P Larkin
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-12
Journal Detail:
Title:  The British journal of ophthalmology     Volume:  94     ISSN:  1468-2079     ISO Abbreviation:  Br J Ophthalmol     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-10     Completed Date:  2010-05-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421041     Medline TA:  Br J Ophthalmol     Country:  England    
Other Details:
Languages:  eng     Pagination:  368-71     Citation Subset:  IM    
Department of Ocular Immunology, UCL Institute of Ophthalmology, London, UK.
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MeSH Terms
Cornea / pathology
Corneal Stroma / pathology
Corneal Topography / methods
Corneal Transplantation*
Diagnostic Techniques, Ophthalmological
Disease Models, Animal
Graft Rejection / pathology,  ultrasonography*
Mice, Inbred A
Mice, Inbred C57BL
Reproducibility of Results
Severity of Illness Index

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