| Use of ultrasonic pachymetry for measurement of changes in corneal thickness in mouse corneal transplant rejection. | |
| | |
MedLine Citation:
|
PMID: 19822919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND/AIMS: Diagnosis of rejection in the mouse model of corneal transplantation is based on subjective judgement of loss of graft transparency. The aims of this study were to (1) evaluate a pachymetry technique to measure changes in mouse corneal thickness and (2) correlate increases in transplant thickness with clinical and histological features of rejection. METHODS: Orthotopic corneal allografts (C57BL/6 strain donor) and syngeneic grafts were performed in A/J mice. Graft transparency was graded and corneal thickness measured by pachymetry on alternate days. Transverse sections of donor cornea excised from eyes representative of clinical opacity grades 1-4 were prepared, photographed, graft section thickness measured and stromal graft-infiltrating cells counted. Intraobserver and interobserver variations in pachymetry were statistically tested. RESULTS: Graft thickness, as measured by pachymetry, increased with each clinical opacity grade. Thickness for opacity grades 0, 1 and 2 was less than 300 microm in all recipients. Graft thickness for grades 3 and 4 was greater than 300 microm in all cases. For measurements up to 400 mum, there was a good correlation between thickness as measured by in vivo pachymetry and in histopathological sections. The mean interobserver bias was -11.35 microm, while the mean intraobserver bias was +3.96 microm. Stromal cellularity increased with increasing corneal thickness up to approximately 300 microm. CONCLUSION: In vivo graft pachymetry provides a new and reliable way to objectively diagnose rejection in the mouse model of corneal transplantation. |
| | |
Authors:
|
T H Flynn; M Ohbayashi; M Dawson; M Siddique; S J Ono; D F P Larkin |
Related Documents
:
|
22137729 - Mtor inhibitor-associated proteinuria in kidney transplant recipients. 18025649 - Conjunctival-limbal autograft, amniotic membrane transplantation, and intraoperative mi... 2488819 - Unusual refractive errors induced by interchanging trephines during penetrating keratop... 10755569 - Icam-1 deficiency suppresses host allosensitization and rejection of mhc-disparate corn... 8460279 - Toxoplasma gondii retinochoroiditis after liver transplantation. 12151269 - Transplantation of cryopreserved human corneas in a xenograft model. 15084179 - Fundamental role for ho-1 in the self-protection of renal allografts. 3893529 - Prolonged survival of allogeneic corneal grafts in rabbits treated with topically appli... 15856479 - Randomized clinical trial of daclizumab induction and delayed introduction of tacrolimu... |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-12 |
Journal Detail:
|
Title: The British journal of ophthalmology Volume: 94 ISSN: 1468-2079 ISO Abbreviation: Br J Ophthalmol Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-03-10 Completed Date: 2010-05-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0421041 Medline TA: Br J Ophthalmol Country: England |
Other Details:
|
Languages: eng Pagination: 368-71 Citation Subset: IM |
Affiliation:
|
Department of Ocular Immunology, UCL Institute of Ophthalmology, London, UK. thomas.flynn@ucl.ac.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cornea / pathology Corneal Stroma / pathology Corneal Topography / methods Corneal Transplantation* Cryopreservation Diagnostic Techniques, Ophthalmological Disease Models, Animal Female Graft Rejection / pathology, ultrasonography* Mice Mice, Inbred A Mice, Inbred C57BL Reproducibility of Results Severity of Illness Index |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effective rehabilitation of reading by training in the technique of eccentric viewing: evaluation of...
Next Document: Fourier domain optical coherence tomographic and auto-fluorescence findings in indeterminate choroid...