| Use of stable isotopically labeled tracers to measure very low density lipoprotein-triglyceride turnover. | |
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MedLine Citation:
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PMID: 11861664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tracer methods for VLDL-TG kinetics vary in their ability to account for the effect of tracer recycling, which can influence the calculation of VLDL-TG fractional catabolic rates (FCRs). We evaluated a novel approach, involving stable isotopically labeled glycerol or palmitate tracers in conjunction with compartmental modeling, for measuring VLDL-TG kinetics in normolipidemic human subjects. When administered as a bolus simultaneously, both tracers provided identical VLDL-TG FCRs when the data were analyzed by a compartmental model that accounted for hepatic lipid tracer recycling, but not by non-compartmental analysis. The model-derived FCR was greater than that determined using a non-compartmental approach, and was 2- to 3-fold higher than that usually reported by using a bolus of radioactive [3H]glycerol. When palmitate tracer was given as a constant infusion, VLDL-TG turnover appeared 5-fold slower, because tracer recycling through hepatic lipid pools could not be resolved with the infusion protocol. We conclude that accounting for tracer recycling, particularly the contribution of hepatic glycerolipid pools, is essential to accurately measure VLDL-TG kinetics, and that bolus injection of stable isotopically labeled glycerol or palmitate tracers in conjunction with compartmental modeling analysis offers a reliable approach for measuring VLDL-TG kinetics. |
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Authors:
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Bruce W Patterson; Bettina Mittendorfer; Nizar Elias; Raj Satyanarayana; Samuel Klein |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of lipid research Volume: 43 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2002 Feb |
Date Detail:
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Created Date: 2002-02-25 Completed Date: 2002-07-31 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 223-33 Citation Subset: IM |
Affiliation:
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Center for Human Nutrition, Department of Internal Medicine, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8031, St. Louis, MO 63110, USA. bpatters@im.wustl.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Carbon Isotopes / chemistry Deuterium / chemistry Female Glycerol / chemistry Humans Infusions, Intravenous Isotope Labeling / methods* Kinetics Lipoproteins, VLDL / blood, metabolism* Male Palmitates / chemistry Triglycerides / blood, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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CA62177/CA/NCI NIH HHS; DK37948/DK/NIDDK NIH HHS; DK56341/DK/NIDDK NIH HHS; RR-00036/RR/NCRR NIH HHS; RR-00954/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Lipoproteins, VLDL; 0/Palmitates; 0/Triglycerides; 56-81-5/Glycerol; 7782-39-0/Deuterium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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