Document Detail


Use of somatic cell nuclear transfer to study meiosis in female cattle carrying a sex-dependent fertility-impairing X-chromosome abnormality.
MedLine Citation:
PMID:  17386019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Animal models have played an important part in establishing our knowledge base on reproduction, development, and the occurrence and impact of chromosome abnormalities. Translocations involving the X chromosome and an autosome are unique in that they elicit sex-dependent infertility, with male carriers rendered sterile by synaptic anomalies during meiosis, whereas female carriers conceive but repeatedly abort. Until now the limited access to relevant fetal oocytes has precluded direct study of meiotic events in female carriers. Because somatic cell nuclear transfer (SCNT) circumvents meiotic problems associated with fertility disturbances in translocation carriers, we used SCNT to generate embryos, fetuses, and calves from a cell line derived from a deceased subfertile X-autosome translocation carrier cow to study the meiotic configurations in carrier oocytes. Data from 33 replicates involving 2470 oocyte-donor-cell complexes were assessed for blastocyst development and of these, 42 blastocysts were transferred to 21 recipients. Fourteen pregnancies were detected on day 35 of gestation. One of these was sacrificed for ovary retrieval on day 94 and three went to term. Features of oocytes from the fetal ovary and from the newborn ovaries were examined. Of the pachytene spreads analyzed, 16%, 82%, and 1.5% exhibited quadrivalent, trivalent/univalent, and bivalent/univalent/univalent structures, respectively, whereas among the diakinesis/metaphase I spreads, 16% ring, 75% chain, and 8.3% bivalent/bivalent configurations were noted, suggesting that the low fertility among female carriers may be related to synaptic errors in a predominant proportion of oocytes. Our results indicate that fibroblasts carrying the X-autosome translocation can be used for SCNT to produce embryos, fetuses, and newborn clones to study such basic aspects of development as meiosis and to generate carriers that cannot easily be reproduced by conventional breeding.
Authors:
Gyu-Jin Rho; Gianfranco Coppola; Jaroslaw Sosnowski; Ramanathan Kasimanickam; Walter H Johnson; Esther Semple; Gabriela F Mastromonaco; Dean H Betts; Thomas G Koch; Scott Weese; Joanne Hewson; Michael Anthony Hayes; Daniel G Kenney; Parvathi K Basrur; William Allan King
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cloning and stem cells     Volume:  9     ISSN:  1536-2302     ISO Abbreviation:  Cloning Stem Cells     Publication Date:  2007  
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-06-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101125444     Medline TA:  Cloning Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  118-29     Citation Subset:  IM    
Affiliation:
Department of Biomedical Science, University of Guelph, Guelph, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cattle Diseases* / genetics
Female
Genetic Diseases, X-Linked* / genetics
Infertility, Female* / genetics,  veterinary
Meiosis*
Models, Biological*
Nuclear Transfer Techniques*
Pregnancy
Sex Chromosome Aberrations* / veterinary

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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