Document Detail

Use of size fractionation of in vitro-activated human B lymphocytes for studies of cell cycle-dependent growth regulation.
MedLine Citation:
PMID:  1899949     Owner:  NLM     Status:  MEDLINE    
Cell cycle progression of in vitro-stimulated human B lymphocytes occurs asynchronously. In order to allow detailed studies of growth control in G1, B cells were stimulated with anti-mu and low molecular weight B-cell growth factor (LMW BCGF) for 50 h and subsequently separated into nine fractions of cells by means of centrifugal elutriation. As judged by volume profiles, activation antigen expression and DNA content, the cells in fractions 1-4 were in early to mid-G1, while fractions 5-7 mainly contained cells in late G1, and fractions 8-9 contained cells mainly in S and G2. Cells in fractions 5-7 had passed the commitment point, as demonstrated by a high spontaneous incorporation of [3H]thymidine when recultured in medium alone. Moreover, S-phase entry of these cells was largely unaffected by exogenous growth-promoting or growth-inhibitory signals. Cells in early (fractions 1-2) and intermediate fractions (fractions 3-4) showed a negligible spontaneous [3H]thymidine incorporation, but a significant proportion of these cells progressed to S phase upon restimulation. Moreover, while IL-4 or the anti-CD40 MoAb G28-5 potently stimulated cells in early and intermediate fractions, the responsiveness of LMW BCGF alone was obtained just prior to the commitment point.
B K Erikstein; P Schwarze; H K Blomhoff; S Funderud; T Stokke; E B Smeland
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  33     ISSN:  0300-9475     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-03-27     Completed Date:  1991-03-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  51-60     Citation Subset:  IM    
Department of Tissue Culture, Norwegian Radium Hospital, Oslo.
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MeSH Terms
B-Lymphocytes / cytology*,  metabolism
Cell Cycle / drug effects,  physiology*
Cell Fractionation / methods
DNA Polymerase II / biosynthesis
Forskolin / pharmacology
Gene Expression
Interferon-gamma / pharmacology
Lymphocyte Activation
Lymphokines / pharmacology
Lymphotoxin-alpha / pharmacology
Transforming Growth Factor beta / pharmacology
Tumor Necrosis Factor-alpha / pharmacology
Reg. No./Substance:
0/Lymphokines; 0/Lymphotoxin-alpha; 0/Transforming Growth Factor beta; 0/Tumor Necrosis Factor-alpha; 0/low molecular weight-B cell growth factor; 66428-89-5/Forskolin; 82115-62-6/Interferon-gamma; EC 2.7.7.-/DNA Polymerase II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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