Document Detail


Use of sea stars to study basic reproductive processes.
MedLine Citation:
PMID:  20536323     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Echinoderms are closely related to chordates and comprise a major group of invertebrate deuterostomes. They are broadcast spawners and as such, each female accumulates millions of eggs and oocytes. These cells are readily isolated, and are often large, clear, and surrounded by accessory cells and extracellular coverings that do not prevent access to the oocyte. Sea star oocytes are stored in prophase of meiosis, and since the natural meiotic stimulus has been identified as 1-methyladenine, these cells can be induced to complete meiotic maturation as individuals, or synchronously en masse. Microinjection and culture of these cells is feasible using quantitative or repetitive methods so that hundreds of oocytes and eggs can be modified each hour. Experimentation on this organism is extensive over a rich history of reproductive and developmental biology so that new investigators can easily incorporate this organism into their repertoire of research. This review will highlight the fundamental protocols to enable a new investigator to perform an array of approaches on this organism, including oocyte isolation, microinjection, and even single cell quantitative PCR.
Authors:
Gary M Wessel; Adrian M Reich; Peter C Klatsky
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Systems biology in reproductive medicine     Volume:  56     ISSN:  1939-6376     ISO Abbreviation:  Syst Biol Reprod Med     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-11     Completed Date:  2010-09-13     Revised Date:  2014-04-24    
Medline Journal Info:
Nlm Unique ID:  101464963     Medline TA:  Syst Biol Reprod Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  236-45     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation
Environmental Exposure
Female
Male
Microinjections
Models, Animal
Oocytes / cytology
Reproduction
Spermatozoa / cytology
Starfish / physiology*
Grant Support
ID/Acronym/Agency:
R01 HD028152/HD/NICHD NIH HHS; T32 GM007601/GM/NIGMS NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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