Document Detail

Use of a ruthenium(III), iron(II), and nickel(II) hexacyanometallate-modified graphite electrode with immobilized oxalate oxidase for the determination of urinary oxalate.
MedLine Citation:
PMID:  11767164     Owner:  NLM     Status:  MEDLINE    
This paper describes the performance of a biosensor with an Ru(III), Ni(II), and Fe(II) hexacyanometallate-modified graphite electrode and immobilized oxalate oxidase for the determination of urinary oxalate. The addition of ruthenium enhances the electrochemical reversibility and chemical stability of the electrocrystallized layer and improves the sensitivity of the biosensor. Hydrogen peroxide, produced by the enzyme-catalyzed oxidation of oxalate, was measured at -50 mV vs an Hg Hg2CI2 3M KCl electrode in a solution of pH 3.6 succinic buffer, 0.1 M KCl, and 5.4mM ethylenediaminetetraacetic acid. The linear concentration range for the determination of oxalate was 0.18-280 microM. The recoveries of added oxalate (10-35 microM) from aqueous solution ranged from 99.5 to 101.7%, whereas from urine samples without oxalate (or with a concentration of oxalate below the detection limit) the recoveries of added oxalate ranged from 91.4 to 106.6%. The oxalate in 24 h urine samples, taken during their daily routine from 35 infants and children, was measured and found to range from 0.6 to 121.7 mg/L. There were no interferences from uric acid, acetylsalicylic acid, and urea in the concentration range investigated, but paracetamol and ascorbic acid did interfere. A good correlation (R2 = 0.9242) was found between values obtained for oxalate in real urine samples by 2 laboratories, with the proposed biosensor and ion chromatography, respectively.
S Milardović; Z Grabarić; V Rumenjak; N Blau; D Milosević
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  84     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2001-12-19     Completed Date:  2002-06-10     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1927-33     Citation Subset:  IM    
University of Zagreb, Faculty of Chemical Engineering and Technology, Croatia.
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MeSH Terms
Biosensing Techniques / methods*,  statistics & numerical data
Chromatography, Ion Exchange / methods
Oxalic Acid / urine*
Sensitivity and Specificity
Reg. No./Substance:
0/Cyanides; 0/Ferrocyanides; 13408-63-4/hexacyanoferrate II; 144-62-7/Oxalic Acid; 7439-89-6/Iron; 7440-02-0/Nickel; 7440-18-8/Ruthenium; 7782-42-5/Graphite; EC 1.-/Oxidoreductases; EC oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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