Document Detail


Use of a ruthenium(III), iron(II), and nickel(II) hexacyanometallate-modified graphite electrode with immobilized oxalate oxidase for the determination of urinary oxalate.
MedLine Citation:
PMID:  11767164     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper describes the performance of a biosensor with an Ru(III), Ni(II), and Fe(II) hexacyanometallate-modified graphite electrode and immobilized oxalate oxidase for the determination of urinary oxalate. The addition of ruthenium enhances the electrochemical reversibility and chemical stability of the electrocrystallized layer and improves the sensitivity of the biosensor. Hydrogen peroxide, produced by the enzyme-catalyzed oxidation of oxalate, was measured at -50 mV vs an Hg Hg2CI2 3M KCl electrode in a solution of pH 3.6 succinic buffer, 0.1 M KCl, and 5.4mM ethylenediaminetetraacetic acid. The linear concentration range for the determination of oxalate was 0.18-280 microM. The recoveries of added oxalate (10-35 microM) from aqueous solution ranged from 99.5 to 101.7%, whereas from urine samples without oxalate (or with a concentration of oxalate below the detection limit) the recoveries of added oxalate ranged from 91.4 to 106.6%. The oxalate in 24 h urine samples, taken during their daily routine from 35 infants and children, was measured and found to range from 0.6 to 121.7 mg/L. There were no interferences from uric acid, acetylsalicylic acid, and urea in the concentration range investigated, but paracetamol and ascorbic acid did interfere. A good correlation (R2 = 0.9242) was found between values obtained for oxalate in real urine samples by 2 laboratories, with the proposed biosensor and ion chromatography, respectively.
Authors:
S Milardović; Z Grabarić; V Rumenjak; N Blau; D Milosević
Related Documents :
10574204 - Determination of urinary orotic acid and uracil by capillary zone electrophoresis.
6194684 - The advanced urine chemistry survey.
6470924 - Identification of sulfur-containing metabolites of m-dichlorobenzene and their disposit...
3055914 - Biological monitoring of styrene: a review.
1988624 - L-methionine uptake by human cerebral cortex: maturation from infancy to old age.
16169624 - Characterization of microorganisms in argentinean honeys from different sources.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  84     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2001-12-19     Completed Date:  2002-06-10     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1927-33     Citation Subset:  IM    
Affiliation:
University of Zagreb, Faculty of Chemical Engineering and Technology, Croatia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / methods*,  statistics & numerical data
Child
Chromatography, Ion Exchange / methods
Cyanides
Ferrocyanides
Graphite
Humans
Infant
Iron
Nickel
Oxalic Acid / urine*
Oxidoreductases
Ruthenium
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Cyanides; 0/Ferrocyanides; 13408-63-4/hexacyanoferrate II; 144-62-7/Oxalic Acid; 7439-89-6/Iron; 7440-02-0/Nickel; 7440-18-8/Ruthenium; 7782-42-5/Graphite; EC 1.-/Oxidoreductases; EC 1.2.3.4/oxalate oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Determination of selenium in marine biological tissues by transverse heated electrothermal atomic ab...
Next Document:  Collection and preparation of sidestream cigarette smoke for trace elemental determinations by graph...