Document Detail


Use of reverse transcriptase polymerase chain reaction (RT-PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird populations.
MedLine Citation:
PMID:  22983878     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of this study was to evaluate a simple molecular method of reverse transcriptase polymerase chain reaction (RT-PCR) to differentiate Newcastle disease virus strains according to their pathogenicity, in order to use it in molecular screening of Newcastle disease virus in poultry and free-living bird populations. Specific primers were developed to differentiate LaSota-LS-(vaccine strain) and Sao Joao do Meriti-SJM-strain (highly pathogenic strain). Chickens and pigeons were experimentally vaccinated/infected for an in vivo study to determine virus shedding in feces. Validation of sensitivity and specificity of the primers (SJM and LS) by experimental models used in the present study and results obtained in the molecular analysis of the primers by BLAST made it possible to generalize results. The development of primers that differentiate the level of pathogenicity of NDV stains is very important, mainly in countries where real-time RT-PCR is still not used as a routine test. These primers were able to determine the presence of the agent and to differentiate it according to its pathogenicity.
Authors:
Adriano de Oliveira Torres Carrasco; Juliana Nogueira Martins Rodrigues; Meire Christina Seki; Fabricio Edgar de Moraes; Jaqueline Raymondi Silva; Edison Luis Durigon; Aramis Augusto Pinto
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-15
Journal Detail:
Title:  Tropical animal health and production     Volume:  -     ISSN:  1573-7438     ISO Abbreviation:  Trop Anim Health Prod     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1277355     Medline TA:  Trop Anim Health Prod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Departamento de Medicina Veterinária, Universidade Estadual do Centro-Oeste, UNICENTRO, Guarapuava, Parana, Brazil, 85040-080, adriano.carrasco@gmail.com.
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