Document Detail

Use of repetitive sequences to identify DNA polymorphisms linked to regA, a developmentally important locus in Volvox.
MedLine Citation:
PMID:  2890555     Owner:  NLM     Status:  MEDLINE    
The regA locus plays a centrally important role in Volvox development by preventing somatic cells from redifferentiating as germ cells; until now, approaches to cloning regA, as a preliminary to molecular analysis of its function, have been lacking. Here a novel approach is described that uses repetitive-sequence probes to rapidly identify restriction fragment length polymorphisms (RFLPs) linked to regA. Genomic DNA was cut with restriction enzymes having 4-base recognition sequences and then electrophoresed long enough to run most fragments off the gel; the remaining long (1- to 20-kb) fragments were resolved into numerous, reproducibly identifiable bands. On Southern blots of such preparations, six repetitive-sequence probes were used to identify 1232 bands, 24% of which were polymorphic between two closely related strains. Ninety-four RFLPs, for which inheritance patterns have been analyzed, fall into 36 "segregation groups," within which no recombination was observed in the limited progeny sample analyzed. Eight RFLPs cosegregated perfectly with alleles at the mating-type (mt) locus. More significantly, four RFLPs exhibited linkage to the regA locus, providing a potential starting place for a chromosome walk designed to clone the locus.
J F Harper; K S Huson; D L Kirk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  1     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1987 Aug 
Date Detail:
Created Date:  1987-12-31     Completed Date:  1987-12-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  573-84     Citation Subset:  IM    
Department of Biology, Washington University, St. Louis, Missouri 63130.
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MeSH Terms
Chromosome Mapping*
Cloning, Molecular
Crosses, Genetic
Eukaryota / cytology,  genetics*,  growth & development
Linkage (Genetics)
Nucleic Acid Hybridization
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length*
Repetitive Sequences, Nucleic Acid
Grant Support

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