| Use of a repetitive mouse B2 element to identify transplanted mouse cells in mouse-chick chimeras. | |
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MedLine Citation:
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PMID: 10094814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monitoring the migrations of cells during embryonic development requires a system in which cells can be identified in situ during locomotion. One promising system involves the generation of chimeras by transplanting mouse cells into chick embryos in ovo to exploit the wealth of mouse genetic variants. The success of this technique relies on the ability to detect individual mouse cells in a chick environment with high specificity. The murine B2 family of short interspersed elements is present in the mouse genome at copy numbers in excess of 10(5), whereas this sequence is absent in the chick genome based on hybridization techniques. This differential of five orders of magnitude produces signals in mouse cells that are easily identified, even in an environment that is predominantly chick. Thus, the B2 repeat probe is highly effective for the purpose of identifying mouse cells in mouse-chick chimeras. |
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Authors:
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R J Bollag; K B Crawford; H Stadt; D Kumiski; M Zdanowicz; C Baptista; V Herlea; M L Kirby |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 248 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-06-03 Completed Date: 1999-06-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 75-8 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Developmental Biology Program, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, 30912, USA. rbollag@mail.mcg.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement / physiology* Cell Transplantation Chick Embryo Mice Repetitive Sequences, Nucleic Acid* |
| Grant Support | |
ID/Acronym/Agency:
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HD17069/HD/NICHD NIH HHS; HD34149/HD/NICHD NIH HHS; HL36059/HL/NHLBI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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