Document Detail

Use of reducing compounds in the cultivation of Azospirillum sp.
MedLine Citation:
PMID:  6419535     Owner:  NLM     Status:  MEDLINE    
Attempts were made to grow the micro-aerophilic N2-fixing bacterium Azospirillum sp. in complete liquid medium by incorporating some reducing agents. Ascorbic acid, glutathione and Na-thioglycollate stimulated while methylene blue inhibited the growth and N2-ase activity. In complete liquid medium, Na-thioglycollate and ascorbic acid increased the N2-ase activity with increasing concentration up to 800 micrograms/ml. With glutathione, growth of the bacterium was increased markedly but N2-ase activity was repressed below 200 micrograms/ml. The possibility of employing these compounds for the cultivation of Azospirillum in complete liquid medium seems to be indicated.
B Venkateswarlu; A V Rao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta microbiologica Hungarica     Volume:  30     ISSN:  0231-4622     ISO Abbreviation:  Acta Microbiol. Hung.     Publication Date:  1983  
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8400270     Medline TA:  Acta Microbiol Hung     Country:  HUNGARY    
Other Details:
Languages:  eng     Pagination:  99-102     Citation Subset:  IM    
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MeSH Terms
Ascorbic Acid / pharmacology*
Bacteria / enzymology,  growth & development*
Culture Media
Glutathione / pharmacology*
Methylene Blue / pharmacology
Nitrogenase / metabolism
Thioglycolates / pharmacology*
Reg. No./Substance:
0/Culture Media; 0/Thioglycolates; 50-81-7/Ascorbic Acid; 61-73-4/Methylene Blue; 70-18-8/Glutathione; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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