Document Detail

Use of red blood cells stored in saline suspension for immediate spin crossmatch.
MedLine Citation:
PMID:  16950678     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to validate a procedure for using red blood cell suspensions stored for up to 10 days in an immediate spin crossmatch. Ten group A and 10 group B SAG-M donor unit segments were opened on day 0. The red cells were divided into 2 tubes and each diluted with saline to a 3% to 5% cell suspension. The cell suspensions were tested using a standard immediate spin technique against pooled group A, group B, and group O plasma each day for 10 days. One set of cells was stored refrigerated as a 3% to 5% cell suspension. Saline was removed from the parallel set of cells before storage. All 480 tests that were expected to be negative were negative. On 2 separate occasions 1+ or 2+ reactions that disappeared before the cell button was completely resuspended were noted. All 960 tests that were expected to be positive were positive. All but 4 results were graded as 3+ or 4+. Four 2+ reactions occurred with 2 units that were typed as A1 negative. Our results indicate that cells stored refrigerated in normal saline as a 3% to 5% cell suspension may be used for immediate spin crossmatch for up to 10 days.
Lisa Denesiuk; Gwen Clarke
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Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Laboratory hematology : official publication of the International Society for Laboratory Hematology     Volume:  12     ISSN:  1080-2924     ISO Abbreviation:  Lab Hematol     Publication Date:  2006  
Date Detail:
Created Date:  2006-09-04     Completed Date:  2006-10-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9615688     Medline TA:  Lab Hematol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  156-9     Citation Subset:  IM    
Dynacare Kasper Medical Laboratories, #200 10150-102 Street, Edmonton, Alberta, Canada.
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MeSH Terms
ABO Blood-Group System / analysis*
Blood Component Transfusion / methods
Blood Grouping and Crossmatching / methods*
Blood Preservation / methods*
Erythrocytes / classification*,  physiology
Sodium Chloride
Time Factors
Reg. No./Substance:
0/ABO Blood-Group System; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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