| Use of recombinant OspC from Borrelia burgdorferi for serodiagnosis of early Lyme disease. | |
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MedLine Citation:
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PMID: 7929767 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infection with Borrelia burgdorferi, the etiologic agent of Lyme disease, is associated with an early and dominant humoral response to the spirochete's 23-kDa outer surface protein C (OspC). We have cloned and expressed OspC as a fusion protein in Escherichia coli and have shown that patient serum samples react with it in an enzyme-linked immunosorbent assay (ELISA) (S. J. Padula, A. Sampieri, F. Dias, A. Szczepanski, and R. W. Ryan, Infect. Immun. 61:5097-5105, 1993). Now we have compared the detection of B. burgdorferi-specific immunoglobulin M antibodies in 74 individuals with culture-positive erythema migrans by a whole-cell ELISA, immunoblot, and the recombinant OspC (rOspC) ELISA. Seventy-six negative controls were also studied. With all of the tests, there was a statistically significant association between the duration of disease and the frequency of a positive result. With the rOspC ELISA, the predictive value of a positive test was 100% and the predictive value of a negative test was 74%. Similar results were obtained with the whole-cell ELISA and with the immunoblot using as the source of test antigen a strain of B. burgdorferi which expresses abundant levels of OspC. We conclude that the use of rOspC in an ELISA is a convenient, readily automated, and easily standardized test for the serodiagnosis of early Lyme disease. |
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Authors:
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S J Padula; F Dias; A Sampieri; R B Craven; R W Ryan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 32 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-11-21 Completed Date: 1994-11-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1733-8 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Connecticut Health Center, Farmington 06030. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Bacterial
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blood* Antigens, Bacterial* Bacterial Outer Membrane Proteins / blood* Borrelia burgdorferi Group / immunology* Enzyme-Linked Immunosorbent Assay / methods Humans Immunoglobulin M / blood* Lyme Disease / blood, diagnosis* Predictive Value of Tests Serologic Tests |
| Grant Support | |
ID/Acronym/Agency:
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R29-AR39361/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Immunoglobulin M; 0/OspC protein |
| Comments/Corrections | |
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