Document Detail


Use of recombinant OspC from Borrelia burgdorferi for serodiagnosis of early Lyme disease.
MedLine Citation:
PMID:  7929767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infection with Borrelia burgdorferi, the etiologic agent of Lyme disease, is associated with an early and dominant humoral response to the spirochete's 23-kDa outer surface protein C (OspC). We have cloned and expressed OspC as a fusion protein in Escherichia coli and have shown that patient serum samples react with it in an enzyme-linked immunosorbent assay (ELISA) (S. J. Padula, A. Sampieri, F. Dias, A. Szczepanski, and R. W. Ryan, Infect. Immun. 61:5097-5105, 1993). Now we have compared the detection of B. burgdorferi-specific immunoglobulin M antibodies in 74 individuals with culture-positive erythema migrans by a whole-cell ELISA, immunoblot, and the recombinant OspC (rOspC) ELISA. Seventy-six negative controls were also studied. With all of the tests, there was a statistically significant association between the duration of disease and the frequency of a positive result. With the rOspC ELISA, the predictive value of a positive test was 100% and the predictive value of a negative test was 74%. Similar results were obtained with the whole-cell ELISA and with the immunoblot using as the source of test antigen a strain of B. burgdorferi which expresses abundant levels of OspC. We conclude that the use of rOspC in an ELISA is a convenient, readily automated, and easily standardized test for the serodiagnosis of early Lyme disease.
Authors:
S J Padula; F Dias; A Sampieri; R B Craven; R W Ryan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  32     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-11-21     Completed Date:  1994-11-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1733-8     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Connecticut Health Center, Farmington 06030.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Bacterial / blood*
Antigens, Bacterial*
Bacterial Outer Membrane Proteins / blood*
Borrelia burgdorferi Group / immunology*
Enzyme-Linked Immunosorbent Assay / methods
Humans
Immunoglobulin M / blood*
Lyme Disease / blood,  diagnosis*
Predictive Value of Tests
Serologic Tests
Grant Support
ID/Acronym/Agency:
R29-AR39361/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Immunoglobulin M; 0/OspC protein
Comments/Corrections

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