| Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present. | |
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MedLine Citation:
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PMID: 12803868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new, rapid assay, based on a single-round, multiplex PCR, can be used to detect Plasmodium falciparum, P. vivax, P. malariae or P. ovale in human blood. The PCR, which targets the conserved 18S small-subunit RNA genes of the parasites, not only permits a malarial infection to be detected but also allows each Plasmodium species present to be identified, even in cases of mixed infection. |
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Authors:
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D Padley; A H Moody; P L Chiodini; J Saldanha |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of tropical medicine and parasitology Volume: 97 ISSN: 0003-4983 ISO Abbreviation: Ann Trop Med Parasitol Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-06-13 Completed Date: 2003-07-03 Revised Date: 2009-05-18 |
Medline Journal Info:
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Nlm Unique ID: 2985178R Medline TA: Ann Trop Med Parasitol Country: England |
Other Details:
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Languages: eng Pagination: 131-7 Citation Subset: IM |
Affiliation:
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Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms EN6 3QG, UK. dpadley@nibsc.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA, Protozoan / analysis Humans Malaria / diagnosis*, parasitology Parasitemia / diagnosis*, parasitology Plasmodium / isolation & purification* Plasmodium falciparum / isolation & purification Plasmodium malariae / isolation & purification Plasmodium ovale / isolation & purification Plasmodium vivax / isolation & purification Polymerase Chain Reaction / methods* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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