Document Detail


Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present.
MedLine Citation:
PMID:  12803868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new, rapid assay, based on a single-round, multiplex PCR, can be used to detect Plasmodium falciparum, P. vivax, P. malariae or P. ovale in human blood. The PCR, which targets the conserved 18S small-subunit RNA genes of the parasites, not only permits a malarial infection to be detected but also allows each Plasmodium species present to be identified, even in cases of mixed infection.
Authors:
D Padley; A H Moody; P L Chiodini; J Saldanha
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of tropical medicine and parasitology     Volume:  97     ISSN:  0003-4983     ISO Abbreviation:  Ann Trop Med Parasitol     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-06-13     Completed Date:  2003-07-03     Revised Date:  2009-05-18    
Medline Journal Info:
Nlm Unique ID:  2985178R     Medline TA:  Ann Trop Med Parasitol     Country:  England    
Other Details:
Languages:  eng     Pagination:  131-7     Citation Subset:  IM    
Affiliation:
Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms EN6 3QG, UK. dpadley@nibsc.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Protozoan / analysis
Humans
Malaria / diagnosis*,  parasitology
Parasitemia / diagnosis*,  parasitology
Plasmodium / isolation & purification*
Plasmodium falciparum / isolation & purification
Plasmodium malariae / isolation & purification
Plasmodium ovale / isolation & purification
Plasmodium vivax / isolation & purification
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/DNA, Protozoan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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