| Use of a radiolabeled monoclonal antibody against E-selectin for imaging of endothelial activation in rheumatoid arthritis. | |
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MedLine Citation:
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PMID: 8702446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the potential of 111In-labeled anti-E-selectin monoclonal antibody (MAb) to image localized endothelial activation in rheumatoid arthritis (RA). METHODS: Fourteen patients with RA were studied after intravenous administration of 111In-labeled F(ab')2 fragments of MAb against the cytokine-inducible endothelial cell activation antigen E-selectin (MAb 1.2B6). To compare uptake of 1.2B6 with that of nonspecific immunoglobulin, 111In-labeled polyclonal human immunoglobulin (HIG) was separately administered to 6 of these patients and the relative uptake of each tracer was determined. RESULTS: Prominent and discrete uptake of the radiolabeled MAb 1.2B6 was clearly visible in inflamed joints of all patients. Compared with 111In-HIG, 111In-1.2B6 provided superior images in terms of sensitivity and image intensity. Furthermore, the distribution of uptake in inflamed joints was different for the 2 tracers, with 1.2B6 showing a more focal localization in synovium. CONCLUSION: This study demonstrates that it is possible to objectively assess E-selectin expression on activated endothelium in vivo in patients with RA, using a radiolabeled MAb. This technique has considerable potential for monitoring disease activity and response to therapy in inflammatory diseases. |
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Authors:
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P T Chapman; F Jamar; E T Keelan; A M Peters; D O Haskard |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Arthritis and rheumatism Volume: 39 ISSN: 0004-3591 ISO Abbreviation: Arthritis Rheum. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1996-09-05 Completed Date: 1996-09-05 Revised Date: 2010-03-24 |
Medline Journal Info:
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Nlm Unique ID: 0370605 Medline TA: Arthritis Rheum Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1371-5 Citation Subset: AIM; IM |
Affiliation:
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Royal Postgraduate Medical School, Hammersmith Hospital, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Antibodies, Monoclonal / diagnostic use, pharmacokinetics Arthritis, Rheumatoid / immunology, radionuclide imaging* E-Selectin / analysis, immunology* Endothelium / chemistry, immunology Female Humans Immunoglobulin Fab Fragments / immunology Immunoglobulins / metabolism Indium Radioisotopes / diagnostic use, pharmacokinetics Male Middle Aged Synovitis / radionuclide imaging |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/E-Selectin; 0/Immunoglobulin Fab Fragments; 0/Immunoglobulins; 0/Indium Radioisotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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