Document Detail

Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid.
MedLine Citation:
PMID:  2187739     Owner:  NLM     Status:  MEDLINE    
The polymerase chain reaction was used to detect Treponema pallidum in specimens of cerebrospinal fluid (CSF), as a means of diagnosing syphilis. Segments of the TmpA and 4D genes were amplified to provide an estimated threshold sensitivity of approximately 65 organisms in 0.5 ml. A spectrum of pathogens known to cause meningitis, and several non-pathogenic treponemes were unreactive. Treponema pertenue, and only one of 30 control specimens of CSF were positive. In contrast, 10 of 19 CSFs from patients being evaluated for latent or tertiary syphilis were positive, as were 7 of 28 specimens from HIV-positive patients.
P E Hay; J R Clarke; R A Strugnell; D Taylor-Robinson; D Goldmeier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  56     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-06-27     Completed Date:  1990-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  233-8     Citation Subset:  IM    
Clinical Research Centre, Harrow, Middlesex, London.
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MeSH Terms
Base Sequence
Blotting, Southern
Cerebrospinal Fluid / microbiology*
DNA, Bacterial / cerebrospinal fluid*,  genetics
Infant, Newborn
Molecular Sequence Data
Polymerase Chain Reaction
Restriction Mapping
Syphilis / cerebrospinal fluid,  diagnosis*
Syphilis, Latent / cerebrospinal fluid,  diagnosis
Treponema pallidum / genetics,  isolation & purification*
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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