| Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid. | |
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MedLine Citation:
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PMID: 2187739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The polymerase chain reaction was used to detect Treponema pallidum in specimens of cerebrospinal fluid (CSF), as a means of diagnosing syphilis. Segments of the TmpA and 4D genes were amplified to provide an estimated threshold sensitivity of approximately 65 organisms in 0.5 ml. A spectrum of pathogens known to cause meningitis, and several non-pathogenic treponemes were unreactive. Treponema pertenue, and only one of 30 control specimens of CSF were positive. In contrast, 10 of 19 CSFs from patients being evaluated for latent or tertiary syphilis were positive, as were 7 of 28 specimens from HIV-positive patients. |
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Authors:
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P E Hay; J R Clarke; R A Strugnell; D Taylor-Robinson; D Goldmeier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS microbiology letters Volume: 56 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-06-27 Completed Date: 1990-06-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 233-8 Citation Subset: IM |
Affiliation:
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Clinical Research Centre, Harrow, Middlesex, London. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blotting, Southern Cerebrospinal Fluid / microbiology* DNA, Bacterial / cerebrospinal fluid*, genetics Humans Infant, Newborn Male Molecular Sequence Data Polymerase Chain Reaction Rabbits Restriction Mapping Syphilis / cerebrospinal fluid, diagnosis* Syphilis, Latent / cerebrospinal fluid, diagnosis Treponema pallidum / genetics, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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