Document Detail


Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid.
MedLine Citation:
PMID:  2187739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The polymerase chain reaction was used to detect Treponema pallidum in specimens of cerebrospinal fluid (CSF), as a means of diagnosing syphilis. Segments of the TmpA and 4D genes were amplified to provide an estimated threshold sensitivity of approximately 65 organisms in 0.5 ml. A spectrum of pathogens known to cause meningitis, and several non-pathogenic treponemes were unreactive. Treponema pertenue, and only one of 30 control specimens of CSF were positive. In contrast, 10 of 19 CSFs from patients being evaluated for latent or tertiary syphilis were positive, as were 7 of 28 specimens from HIV-positive patients.
Authors:
P E Hay; J R Clarke; R A Strugnell; D Taylor-Robinson; D Goldmeier
Related Documents :
20347569 - Evaluation of cerebrospinal fluid adenosine deaminase activity in hiv-seropositive subj...
6155449 - Isoelectric focusing and quantitative estimation of cerebrospinal fluid and serum igg i...
8759489 - The spectrum of meningitis in a population with high prevalence of hiv disease.
8036469 - Interferon-gamma in cerebrospinal fluid from patients with viral and bacterial meningitis.
7449789 - Early detection of bacterial antigens by counterimmunoelectrophoresis.
15765809 - Serum and cerebrospinal fluid levels of interleukin-18 in human immunodeficiency virus ...
24816919 - Association between mir-146ag>c and mir-196a2c>t polymorphisms and the risk of hepatoce...
25246359 - Exploratory trial of ombitasvir and abt-450/r with or without ribavirin for hcv genotyp...
10695229 - Knowledge and practices about hiv transmission among barbers of nagpur city.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  56     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-06-27     Completed Date:  1990-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  233-8     Citation Subset:  IM    
Affiliation:
Clinical Research Centre, Harrow, Middlesex, London.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blotting, Southern
Cerebrospinal Fluid / microbiology*
DNA, Bacterial / cerebrospinal fluid*,  genetics
Humans
Infant, Newborn
Male
Molecular Sequence Data
Polymerase Chain Reaction
Rabbits
Restriction Mapping
Syphilis / cerebrospinal fluid,  diagnosis*
Syphilis, Latent / cerebrospinal fluid,  diagnosis
Treponema pallidum / genetics,  isolation & purification*
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Transcriptional control of SSL1, a gene controlling alpha-specific inactivation of a-factor in Sacch...
Next Document:  Adherence of Candida albicans to components of the subendothelial extracellular matrix.