Document Detail


Use of polymerase chain reaction in analysing recombinant cDNA clones encoding storage proteins of chickpea Cicer arietinum L.
MedLine Citation:
PMID:  9055657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and reliable method was undertaken for the use of polymerase chain reaction in analyzing cDNA clones. Amplification was done of the inserts from positive legumin clones isolated from a cDNA library constructed from developing chickpea cotyledons in the expression vector, gt11. Amplification was made simple by using oligonucleotide primers which allowed convenient sizing, subcloning and sequencing of inserts by di-deoxy chain termination method. This simple method may provide opportunity to isolate large number of agronomically important genes from gene libraries.
Authors:
S Saha; R Kansal; K R Koundal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Indian journal of experimental biology     Volume:  34     ISSN:  0019-5189     ISO Abbreviation:  Indian J. Exp. Biol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-06-05     Completed Date:  1997-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0233411     Medline TA:  Indian J Exp Biol     Country:  INDIA    
Other Details:
Languages:  eng     Pagination:  1019-25     Citation Subset:  IM    
Affiliation:
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / genetics
DNA, Plant / genetics
Fabaceae / genetics*
Molecular Sequence Data
Plant Proteins / genetics*
Plants, Medicinal*
Polymerase Chain Reaction
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Plant; 0/Plant Proteins; 0/legumin protein, plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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