Document Detail


Use of plant cell cultures to study graminicide effects on lipid metabolism.
MedLine Citation:
PMID:  12809713     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Graminicides belonging to the cyclohexanedione and aryloxyphenoxypropionate classes are well established to act by disrupting acyl lipid biosynthesis via specific inhibition of acetyl-CoA carboxylase. Species of grass inherently resistant to such herbicides, or biotypes of grassy weed species which display acquired resistance to recommended rates of graminicide application, are known to possess an altered plastidic multifunctional acetyl-CoA carboxylase showing reduced sensitivity to these herbicides in vitro. Studies reported here demonstrate that cell suspension cultures of maize, a graminicide-sensitive species and Poa annua, a graminicide-insensitive species, display a similar differential sensitivity of acyl lipid biosynthesis as tissue from corresponding intact plants. Acyl lipid biosynthesis in P. annua can be inhibited if sufficiently high concentrations of graminicide are used. The major plastidic form and the minor cytosolic forms of acetyl-CoA carboxylase were successfully purified from maize cell suspensions, were compared to those from leaf tissue and were shown to be differentially inhibited by graminicides in a similar manner to their counterparts from leaf tissue. These studies demonstrate that cell suspensions are useful for studying the mode of action of graminicides, especially in view of the limited amount of material obtainable from many grassy species which are very fine-growing.
Authors:
Lindsey J Price; Derek Herbert; David J Cole; John L Harwood
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  63     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-17     Completed Date:  2003-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  533-41     Citation Subset:  IM    
Affiliation:
School of Biosciences, Cardiff University, PO Box 911, Cardiff CF10 3US, UK.
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MeSH Terms
Descriptor/Qualifier:
Acetyl-CoA Carboxylase / antagonists & inhibitors,  metabolism
Cell Culture Techniques
Cyclohexanones / pharmacology*
Lipid Metabolism*
Plant Leaves / drug effects,  enzymology,  metabolism
Poa / cytology,  drug effects*,  enzymology,  metabolism*
Propionic Acids / pharmacology*
Quinoxalines / pharmacology*
Species Specificity
Zea mays / cytology,  drug effects*,  enzymology,  metabolism*
Chemical
Reg. No./Substance:
0/Cyclohexanones; 0/Propionic Acids; 0/Quinoxalines; 0/quizalofop; 74051-80-2/sethoxydim; 76578-14-8/quizalofop-ethyl; EC 6.4.1.2/Acetyl-CoA Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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