| Use of peptide nucleic acid-fluorescence in situ hybridization for definitive, rapid identification of five common Candida species. | |
| | |
MedLine Citation:
|
PMID: 17804657 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We investigated a 2.5-h peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay with five Candida species-specific probes to identify Candida colonies and compared it to standard 2-h to 5-day phenotypic identification methods. Suspensions were made and slides were prepared and read for fluorescence per the manufacturer's instructions. Sensitivity was 99% (109/110), and specificity was 99% (129/130). PNA-FISH can rapidly identify those Candida species isolated most frequently. |
| | |
Authors:
|
Megan E Reller; Amanda B Mallonee; Nicole P Kwiatkowski; William G Merz |
Related Documents
:
|
398767 - Pathways of assimilatory sulphate reduction in plants and microorganisms. 2984127 - Radiation-induced decomposition of the purine bases within dna and related model compou... 18962147 - Determination of pimozide in oral preparations by spectrofluorometry. 223127 - Fluoride ion catalyzed alkylation of nucleic acid derivatives using trialkyl phosphates... 9703897 - Differential effects and transport kinetics of ascorbate derivatives in leukemic cell l... 18589567 - Prevalence of anemia and deficiencies of iron, folic acid and vitamin b12 in an indigen... |
Publication Detail:
|
Type: Journal Article Date: 2007-09-05 |
Journal Detail:
|
Title: Journal of clinical microbiology Volume: 45 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2007 Nov |
Date Detail:
|
Created Date: 2007-11-06 Completed Date: 2007-12-20 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 3802-3 Citation Subset: IM |
Affiliation:
|
Medical Microbiology Division, Department Pathology, Meyer B1-193, The Johns Hopkins Hospital, Baltimore, MD 21287-7093, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Candida
/
isolation & purification* Humans In Situ Hybridization, Fluorescence / methods* Nucleic Acid Probes / genetics* Peptide Nucleic Acids / genetics* |
| Chemical | |
Reg. No./Substance:
|
0/Nucleic Acid Probes; 0/Peptide Nucleic Acids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Feasibility of a molecular screening method for detection of Salmonella enterica and Campylobacter j...
Next Document: Tracing shigatoxigenic Escherichia coli O103, O145, and O174 infections from farm residents to cattl...