| Use of a penetratin-linked peptide in Dictyostelium. | |
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MedLine Citation:
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PMID: 16757799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The plasma membrane is an effective barrier to most macromolecules and hydrophilic molecules. Remarkably, a class of positively charged cell-penetrating peptides (CPPs) has been discovered that can translocate themselves and associated cargoes into the cytoplasm. These have been used to carry oligopeptide- and oligonucleotide-based inhibitors into mammalian cells. A recent report indicates that the same CPPs are internalized by plant protoplasts, suggesting that this may be a universal phenomenon. We report here that the prototypical CPP, penetratin, enters cells of the free-living amoebae Dictyostelium discoideum. To investigate the functionality of this technology, we fused the penetratin sequence to PKI, a peptide inhibitor of the cAMP-dependent protein kinase (PKA). Consistent with its PKA inhibitory action, Penetratin-PKI blocked aggregation in wild-type cells and, at appropriate concentrations, rescued the phenotype of a Dictyostelium mutant that has constitutively high PKA activity. This technology offers an effective method for delivery of oligopeptides and oligonucleotides into Dictyostelium. |
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Authors:
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W Jonathan Ryves; Adrian J Harwood |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular biotechnology Volume: 33 ISSN: 1559-0305 ISO Abbreviation: Mol. Biotechnol. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-07 Completed Date: 2010-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 123-32 Citation Subset: IM |
Affiliation:
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Cardiff School of Biosciences, Cardiff, CF10 3US, Wales, UK. ryveswj@cardiff.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carrier Proteins / genetics, metabolism* Cell Aggregation Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors Dictyostelium / cytology, enzymology, metabolism* Mutation / genetics Peptides / genetics, metabolism*, pharmacology Spores, Protozoan / growth & development |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Peptides; 0/penetratin; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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