Document Detail

Use of paraffin-embedded tissue for identification of Saccharomyces cerevisiae in a baker's lung nodule by fungal PCR and nucleotide sequencing.
MedLine Citation:
PMID:  15184487     Owner:  NLM     Status:  MEDLINE    
A 40-year-old healthy male employed in a bakery presented with a single lung nodule and underwent investigations to rule out pulmonary carcinoma. Biopsy was positive for yeast cells, which did not match common fungal pathogens. PCR assay of paraffin-embedded tissue and nucleotide sequencing with ribosomal ITS1-ITS2 universal primers revealed the presence of Saccharomyces cerevisiae.
Ping Ren; Sundara Sridhar; Vishnu Chaturvedi
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  42     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-08     Completed Date:  2004-07-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2840-2     Citation Subset:  IM    
Mycology Laboratory, Wadsworth Center, New York State Department of Health, SUNY School of Public Health, University at Albany, Albany, NY 12201-2002, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF500488;  AY525600
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MeSH Terms
Base Sequence
Lung / microbiology*
Molecular Sequence Data
Occupational Exposure
Paraffin Embedding
Polymerase Chain Reaction / methods*
Saccharomyces cerevisiae / isolation & purification*
Sequence Analysis, DNA

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