Document Detail


Use of outer surface protein repeat regions for improved genotyping of Staphylococcus epidermidis.
MedLine Citation:
PMID:  17202277     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcus epidermidis is an important nosocomial pathogen, but little is known of its epidemiology. Accurate, reproducible typing systems would greatly improve epidemiologic investigations of S. epidermidis. The sequence-based typing technique most recently evaluated, multilocus sequence typing (MLST), often lacks discrimination and can be expensive. PCR and sequence-based analyses of the serine-aspartate repeat region of sdrG (Fbe) and the repeat region of the accumulation-associated protein gene (aap) were evaluated for the ability to discriminate among previously well-characterized S. epidermidis clinical isolates. Forty-eight strains were investigated, with sdrG found in 100% and aap found in 79% of all strains tested. Both genes demonstrated PCR product size and nucleotide sequence variation. Each system by itself gave an index of discrimination similar in value to that of MLST (0.924 and 0.953 compared to 0.96), but discrimination was further improved when combinations of the three systems were used. We conclude that typing systems using amino acid and nucleotide repeat regions of the S. epidermidis surface proteins SdrG and Aap show promise as typing tools and should be investigated using a larger panel of clinically relevant isolates.
Authors:
Alastair B Monk; Gordon L Archer
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2007-01-03
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  45     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-05     Completed Date:  2007-05-10     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  730-5     Citation Subset:  IM    
Affiliation:
Division of Infectious Diseases, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, Virginia 23298, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Aspartic Acid / genetics
Bacterial Outer Membrane Proteins / chemistry,  genetics*
Bacterial Proteins / chemistry,  genetics*
Bacterial Typing Techniques*
Base Sequence
Carrier Proteins / chemistry,  genetics*
Genotype
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid / genetics*
Sequence Analysis, DNA
Serine / genetics
Staphylococcus epidermidis / classification*,  genetics
Grant Support
ID/Acronym/Agency:
5R01AI35705-13/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Fbe protein, bacteria; 56-45-1/Serine; 56-84-8/Aspartic Acid
Comments/Corrections

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