Document Detail


Use of optical density detection times to assess the effect of acetic acid on single-cell kinetics.
MedLine Citation:
PMID:  16950913     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The growth of Listeria innocua at different acetic acid concentrations (0 to 2,000 ppm) was monitored by optical density measurements in a Bioscreen (Labsystems, Vantaa, Finland). The generated populations came from low inocula that were obtained by serial dilution. A new method to estimate both the growth rate and the lag time of single cells from the detection times (time to reach an optical density of 0.11) was developed. It assumes that the single-cell lag times follow a gamma distribution and takes into account the randomness of the inoculation level. (The initial cell number per well was assumed to follow a Poisson distribution.) In this way, relatively small numbers of replicates are sufficient to obtain a robust estimation of the distribution of single-cell lag times. The results were validated with plate count experiments. It was found that logarithms of both the growth rates and of population lag times increased linearly with the acetic acid concentration. The logarithm of the scale parameter of the gamma distribution of the single-cell lag times also increased linearly with the acetic acid concentration irrespective of the phase of the inoculum.
Authors:
A Métris; S M George; J Baranyi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-05     Completed Date:  2006-11-14     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6674-9     Citation Subset:  IM    
Affiliation:
Institute of Food Research, NRP, Norwich NR4 7UA, United Kingdom. aline.metris@bbsrc.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / pharmacology*
Cell Count
Colony Count, Microbial / statistics & numerical data
Finland
Kinetics
Listeria / cytology,  drug effects*
Time Factors
Chemical
Reg. No./Substance:
64-19-7/Acetic Acid
Comments/Corrections

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