| Use of optical density detection times to assess the effect of acetic acid on single-cell kinetics. | |
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MedLine Citation:
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PMID: 16950913 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The growth of Listeria innocua at different acetic acid concentrations (0 to 2,000 ppm) was monitored by optical density measurements in a Bioscreen (Labsystems, Vantaa, Finland). The generated populations came from low inocula that were obtained by serial dilution. A new method to estimate both the growth rate and the lag time of single cells from the detection times (time to reach an optical density of 0.11) was developed. It assumes that the single-cell lag times follow a gamma distribution and takes into account the randomness of the inoculation level. (The initial cell number per well was assumed to follow a Poisson distribution.) In this way, relatively small numbers of replicates are sufficient to obtain a robust estimation of the distribution of single-cell lag times. The results were validated with plate count experiments. It was found that logarithms of both the growth rates and of population lag times increased linearly with the acetic acid concentration. The logarithm of the scale parameter of the gamma distribution of the single-cell lag times also increased linearly with the acetic acid concentration irrespective of the phase of the inoculum. |
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Authors:
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A Métris; S M George; J Baranyi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-01 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 72 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-05 Completed Date: 2006-11-14 Revised Date: 2010-09-15 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 6674-9 Citation Subset: IM |
Affiliation:
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Institute of Food Research, NRP, Norwich NR4 7UA, United Kingdom. aline.metris@bbsrc.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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pharmacology* Cell Count Colony Count, Microbial / statistics & numerical data Finland Kinetics Listeria / cytology, drug effects* Time Factors |
| Chemical | |
Reg. No./Substance:
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64-19-7/Acetic Acid |
| Comments/Corrections | |
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