| Use of nasal cells in micronucleus assays and other genotoxicity studies. | |
| | |
MedLine Citation:
|
PMID: 21164207 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Genotoxicity experiments with exfoliated nasal mucosa cells are a promising minimally invasive approach for the detection of DNA-damaging compounds in ambient air. Results of single cell gel electrophoresis (SCGE) assays with individual cells and organ cultures from bioptic material show that DNA damage caused by compounds such as nitrosamines, polycyclic aromatic hydrocarbons and pesticides can be detected. Biochemical studies indicate that enzymes involved in the metabolism of environmental mutagens are represented in nasal cells. Several protocols for experiments with nasal cells have been developed and it was shown that formaldehyde, metals, styrene and crystalline silica induce DNA damage in SCGE and/or in micronucleus studies; furthermore, it was also found that polluted urban air causes DNA instability in nasal epithelial cells. Comparisons of these data with results obtained in lymphocytes and buccal cells indicate that nasal cells are in general equally sensitive. Broad variations in the baseline levels, differences of results obtained in various studies as well as the lack of information concerning the impact of confounding factors on the outcome of experiments with these cells indicate the need for further standardisation of the experimental protocols. |
| | |
Authors:
|
Siegfried Knasmueller; Nina Holland; Georg Wultsch; Barbara Jandl; Sema Burgaz; Miroslav Misík; Armen Nersesyan |
Related Documents
:
|
16118057 - Satellite cells, myoblasts and other occasional myogenic progenitors: possible origin, ... 11467787 - Different response of satellite cells in the kinetics of myogenic regulatory factors an... 11121437 - Expression of cd34 and myf5 defines the majority of quiescent adult skeletal muscle sat... 7312707 - Chordoma of the spinal cord in a f344 rat. 1934057 - Microsurgical removal of centrosomes blocks cell reproduction and centriole generation ... 2598817 - Roles of neuroepithelial cell rearrangement and division in shaping of the avian neural... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Mutagenesis Volume: 26 ISSN: 1464-3804 ISO Abbreviation: Mutagenesis Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8707812 Medline TA: Mutagenesis Country: England |
Other Details:
|
Languages: eng Pagination: 231-8 Citation Subset: IM |
Affiliation:
|
Institute of Cancer Research, Department of Internal Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria. siegfried.knasmueller@meduniwien.ac.at |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Micronucleus assays in rodent tissues other than bone marrow.
Next Document: The HUMN and HUMNxL international collaboration projects on human micronucleus assays in lymphocytes...