| Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions: a role for T-cadherin in tumor angiogenesis. | |
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MedLine Citation:
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PMID: 17765896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study addresses establishment of an "in vitro" melanoma angiogenesis model using multicellular tumor spheroids (MCTS) of differentiated (HBL) or undifferentiated (NA8) melanoma cell lines. DNA microarray assay and qRT-PCR indicated upregulation of pro-angiogenic factors IL-8, VEGF, Ephrin A1 and ANGPTL4 in NA8-MCTSs (vs. monolayers) whereas these were absent in MCTS and monolayer cultures of HBL. Upon co-culture with endothelial cell line HMEC-1 NA8-MCTS attract, whereas HBL-MCTS repulse, HMEC-1. Overexpression of T-cadherin in HMEC-1 leads to their increased invasion and network formation within NA8-MCTS. Given an appropriate angiogenic tumor microenvironment, T-cadherin upregulation on endothelial cells may potentiate intratumoral angiogenesis. |
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Authors:
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Sourabh Ghosh; Manjunath B Joshi; Danila Ivanov; Chantal Feder-Mengus; Giulio C Spagnoli; Ivan Martin; Paul Erne; Therese J Resink |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-28 |
Journal Detail:
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Title: FEBS letters Volume: 581 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-09-11 Completed Date: 2008-01-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 4523-8 Citation Subset: IM |
Affiliation:
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ICFS, Departments of Surgery and Research, University Hospital, Basel, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cadherins
/
genetics,
metabolism,
physiology* Cell Line Cell Line, Tumor Endothelial Cells / cytology, metabolism* Gene Expression Regulation, Neoplastic Humans Melanoma / genetics, metabolism, pathology Microscopy, Confocal Neovascularization, Pathologic / genetics, metabolism, physiopathology* Reverse Transcriptase Polymerase Chain Reaction Spheroids, Cellular / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cadherins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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