| Use of monoclonal antibodies in diagnosis of paracoccidioidomycosis: new strategies for detection of circulating antigens. | |
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MedLine Citation:
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PMID: 9399534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The precise diagnosis of paracoccidioidomycosis, in most cases, is established by direct methods and indirect immunological tests. The latter method is reliant on the identification of the host's humoral responses, which are usually impaired or absent in patients with severe juvenile forms of the disease and in immunocompromised patients. Determining disease activity or assessing treatment responses by measuring antibody levels is difficult, since antibody titer may remain elevated or persist at stationary levels, even in the presence of clinical improvement. Consequently, there is a need for alternative tests aimed at the identification of circulating antigens. A modification of the standard hybridoma production method was used to raise a panel of murine monoclonal antibodies (MAbs) against the yeast form of Paracoccidioides brasiliensis. Of these, MAb PIB, directed against an 87-kDa determinant, was used to develop an inhibition ELISA (inh-ELISA) capable of detecting as little as 5.8 ng of circulating antigen per ml of serum. Sera from 46 patients with paracoccidioidomycosis or other mycoses and sera from healthy individuals were evaluated by the inh-ELISA; overall sensitivity was 80.4% (37 of 46 paracoccidioidomycosis patients tested positive), and specificity compared with that of normal controls from areas of endemicity was 81.4%. The inh-ELISA detected circulating antigen in 100% of patients with the acute form of paracoccidioidomycosis and in 83.3 and 60% of patients with the chronic multifocal and unifocal forms of paracoccidioidomycosis according to the patients' clinical presentation. These results indicate that the inh-ELISA with MAb PIB is effective in the detection of circulating antigen and that this test may be useful for monitoring responses to treatment and establishing disease prognoses. |
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Authors:
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B L Gómez; J I Figueroa; A J Hamilton; B Ortiz; M A Robledo; R J Hay; A Restrepo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 35 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-02-05 Completed Date: 1998-02-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3278-83 Citation Subset: IM |
Affiliation:
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Corporación para Investigaciones Biológicas, Medellín, Colombia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Fungal
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diagnostic use* Antibodies, Monoclonal / diagnostic use* Antigens, Fungal / blood*, chemistry Enzyme-Linked Immunosorbent Assay / methods, statistics & numerical data Evaluation Studies as Topic Humans Molecular Weight Mycology / methods, statistics & numerical data Paracoccidioides / immunology* Paracoccidioidomycosis / diagnosis*, immunology, microbiology* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Fungal; 0/Antibodies, Monoclonal; 0/Antigens, Fungal |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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