Document Detail

Use of modified Doehlert-type experimental design in optimization of a hybrid electrospray ionization ion trap time-of-flight mass spectrometry technique for glutathione determination.
MedLine Citation:
PMID:  23280982     Owner:  NLM     Status:  MEDLINE    
RATIONALE: The capabilities of modified non-spherical Doehlert-type experimental designs to optimize the performance of a hybrid mass spectrometer were investigated in this paper for the first time. The optimization process was completed in three successive steps with groups of variables avoiding any univariable approach. Glutathione (GSH, reduced) was selected as a very interesting analyte since it is considered to be one of the most abundant tripeptides in human organism and its action against xenobiotics and oxidative radicals is well known.
METHODS: In particular, a tandem technique based on sequential ion management by an ion trap followed by a time-of-flight mass analyzer (ITTOFMS) was introduced commercially in recent years and investigated for glutathione determination. Glutathione was injected in a stream of a typical mobile phase used in liquid chromatography (LC) and analyzed after electrospray ionization (ESI) in tandem MS.
RESULTS: The three main steps of the LC/MS system, namely the LC mobile phase, the ESI interface and the MS analyzer, were independently optimized in terms of maximum sensitivity. In this context quadratic models were found and their prediction power was evaluated. A calibration study was performed at default and optimum conditions in order to quantitatively estimate the sensitivity enhancement of the employed technique for this analyte. Satisfactory precision (RSD 5.7%) and detectability (LOD 0.07 µmol L(-1) ) were achieved.
CONCLUSIONS: The results highlighted the possibilities offered by the employment of multifactorial optimization towards the improvement of performance of tandem mass spectrometry techniques.
George A Zachariadis; Erwin Rosenberg
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  27     ISSN:  1097-0231     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-06-03     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  489-99     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, 54124, Thessaloniki, Greece.
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MeSH Terms
Acetonitriles / chemistry
Glutathione / analysis*,  chemistry
Limit of Detection
Regression Analysis
Reproducibility of Results
Research Design
Spectrometry, Mass, Electrospray Ionization / methods*
Tandem Mass Spectrometry / methods
Reg. No./Substance:
0/Acetonitriles; 70-18-8/Glutathione; Z072SB282N/acetonitrile

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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