Document Detail


Use of microwave fixation in the preparation of cell cultures for observation with the scanning electron microscope.
MedLine Citation:
PMID:  2250187     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper describes a method for primary fixation of cultured cells for scanning (SEM) and transmission (TEM) electron microscopy using microwaves alone. This method of fixation takes 8 seconds and is therefore quicker and less expensive than conventional fixation techniques. The preservation of cell morphology is excellent and cultures of mammalian immune system cells and peripheral nervous tissue have been examined using this fixation.
Authors:
K Argall; P Armati
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of electron microscopy technique     Volume:  16     ISSN:  0741-0581     ISO Abbreviation:  J Electron Microsc Tech     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-01-16     Completed Date:  1991-01-16     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8502171     Medline TA:  J Electron Microsc Tech     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  347-50     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, University of Sydney, New South Wales, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Cell Communication
Cells, Cultured
Fibroblasts / ultrastructure*
Ganglia, Spinal / cytology
Microscopy, Electron
Microscopy, Electron, Scanning / methods*
Microwaves*
Preservation, Biological
Rats
Rats, Inbred Lew
Schwann Cells / ultrastructure*
T-Lymphocytes / ultrastructure*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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