| Use of microwave fixation in the preparation of cell cultures for observation with the scanning electron microscope. | |
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MedLine Citation:
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PMID: 2250187 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper describes a method for primary fixation of cultured cells for scanning (SEM) and transmission (TEM) electron microscopy using microwaves alone. This method of fixation takes 8 seconds and is therefore quicker and less expensive than conventional fixation techniques. The preservation of cell morphology is excellent and cultures of mammalian immune system cells and peripheral nervous tissue have been examined using this fixation. |
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Authors:
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K Argall; P Armati |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of electron microscopy technique Volume: 16 ISSN: 0741-0581 ISO Abbreviation: J Electron Microsc Tech Publication Date: 1990 Dec |
Date Detail:
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Created Date: 1991-01-16 Completed Date: 1991-01-16 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8502171 Medline TA: J Electron Microsc Tech Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 347-50 Citation Subset: IM |
Affiliation:
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School of Biological Sciences, University of Sydney, New South Wales, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Cell Communication Cells, Cultured Fibroblasts / ultrastructure* Ganglia, Spinal / cytology Microscopy, Electron Microscopy, Electron, Scanning / methods* Microwaves* Preservation, Biological Rats Rats, Inbred Lew Schwann Cells / ultrastructure* T-Lymphocytes / ultrastructure* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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