Document Detail

Use of micromachined probes for the recording of cardiac electrograms in isolated heart tissues.
MedLine Citation:
PMID:  15018966     Owner:  NLM     Status:  MEDLINE    
Micromachined probes, with iridium (Ir) microelectrodes on silicon shanks, were evaluated to assess their suitability for cardiac electrogram recording. The electrochemical activation (anodic oxidation) procedure for the circular Ir microelectrode was investigated using the square wave potential according to the electrode size, number of cycles, and cathodic-anodic potential level of the square wave. Increase in the charge storage capacity was pronounced either in smaller electrodes or with higher potential level of the square wave. The electrode impedance reduced in a similar manner with increasing number of cycle irrespective of the electrode size. With either lower potential level (-0.70/+0.60 V) or smaller number of cycle (200 cycles) than those for the activation of stimulating electrode, the likelihood of overactivation of the recording microelectrode can be minimized. These anodic IrOx film (AIROF) microelectrodes were used for the recording of extracellular electrograms in two different ex vivo cardiac tissue preparations. A single-shank microprobe was applied to the left ventricle of a mouse heart. Both the spontaneous and paced transmural responses propagating between epicardium and endocardium were obtained. Longitudinal cardiac wavefronts propagating along the rabbit papillary muscle were also recorded with a unique multiple-shank design. The measured mean amplitude and the propagation velocity of the extracellular voltage were 12.2 +/- 1.8 mV and 58.9 +/- 2.2 cm/s, respectively (n = 27). These microprobes with precisely defined electrode spacing make a useful tool for the spatial and temporal mapping of electrical properties in isolated heart tissues ex vivo.
Chang-Soo Kim; Stefan Ufer; Christopher M Seagle; Connie L Engle; H Troy Nagle; Timothy A Johnson; Wayne E Cascio
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  19     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-15     Completed Date:  2004-10-21     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1109-16     Citation Subset:  IM    
Department of Electrical & Computer Engineering, 1870 Miner Circle, University of Missouri-Rolla, Rolla, MO 65409, USA.
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MeSH Terms
Biosensing Techniques*
Electrocardiography / methods*
Heart / physiology*
Membrane Potentials
Myocardium / metabolism
Grant Support
Reg. No./Substance:

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