Document Detail

Use of a medium buffered with N-hydroxyethylpiperazine-N-ethanesulfonate (HEPES) in intracytoplasmic sperm injection procedures is detrimental to the outcome of in vitro fertilization.
MedLine Citation:
PMID:  16600227     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: This study was conducted to determine whether N-hydroxyethylpiperazine-N-ethanesulfonate (HEPES)-buffered medium used for the microinjection of sperm into oocytes may be detrimental for the embryo. DESIGN: Controlled randomized study. SETTING: Private IVF center. PATIENT(S): Women (n = 708) undergoing ICSI. INTERVENTION(S): The women were randomized into two study groups: 2,204 oocytes from 357 women were treated using a medium buffered with bicarbonate without HEPES during the ICSI procedure, and 2,168 oocytes from 351 women were treated using a medium buffered with HEPES during the ICSI procedure. MAIN OUTCOME MEASURE(S): Fertilization rate, degeneration rate, triploid rate, cleavage rate, embryo quality, pregnancy rate, implantation rate, and abortion rate. RESULT(S): Oocytes treated with a HEPES-buffered medium showed a statistically significant higher rate of triploid and degenerated oocytes after fertilization with ICSI compared with oocytes treated with a medium without HEPES. The embryos obtained from oocytes microinjected with a HEPES-buffered medium showed a statistically significant higher rate of highly fragmented embryos compared with the controls. Pregnancy rate and implantation rate were statistically significantly lower in the patient group with oocytes treated with the HEPES-buffered medium. The other parameters evaluated did not show any statistically significant differences. CONCLUSION(S): Our study showed that the use of media buffered with HEPES, during the microinjection of sperm into the oocytes, is detrimental for IVF outcome and should be avoided.
Francesco Morgia; Monica Torti; Monica Montigiani; Claudio Piscitelli; Annalise Giallonardo; Mauro Schimberni; Pierluigi Giannini; Marco Sbracia
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial     Date:  2006-04-05
Journal Detail:
Title:  Fertility and sterility     Volume:  85     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-01     Completed Date:  2006-05-23     Revised Date:  2007-02-28    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1415-9     Citation Subset:  IM    
Bioroma, Center of Assisted Reproduction, Paideia Hospital, Rome, Italy.
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MeSH Terms
Culture Media / chemistry
Fertilization in Vitro / statistics & numerical data
HEPES / administration & dosage*,  chemistry
Infertility, Female / epidemiology*,  therapy*
Outcome Assessment (Health Care)
Pregnancy Outcome
Sperm Injections, Intracytoplasmic / statistics & numerical data*
Treatment Failure
Treatment Outcome
Reg. No./Substance:
0/Buffers; 0/Culture Media; 7365-45-9/HEPES
Comment In:
Fertil Steril. 2007 Feb;87(2):451; author reply 451   [PMID:  17276155 ]
Fertil Steril. 2006 Dec;86(6):1804; author reply 1804-5   [PMID:  17055500 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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