| Use of light absorbers to alter optical interrogation with epi-illumination and transillumination in three-dimensional cardiac models. | |
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MedLine Citation:
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PMID: 16674209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cardiac optical mapping currently provides 2-D maps of transmembrane voltage-sensitive fluorescence localized near the tissue surface. Methods for interrogation at different depths are required for studies of arrhythmias and the effects of defibrillation shocks in 3-D cardiac tissue. We model the effects of coloading with a dye that absorbs excitation or fluorescence light on the radius and depth of the interrogated region with specific illumination and collection techniques. Results indicate radii and depths of interrogation are larger for transillumination versus epi-illumination, an effect that is more pronounced for broad-field excitation versus laser scanner. Coloading with a fluorescence absorber lessens interrogated depth for epi-illumination and increases it for transillumination, which is confirmed with measurements using transillumination of heart tissue slices. Coloading with an absorber of excitation light consistently decreases the interrogated depths. Transillumination and coloading also decrease the intensities of collected fluorescence. Thus, localization can be modified with wavelength-specific absorbers at the expense of a reduction in fluorescence intensity. |
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Authors:
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Venkat K Ramshesh; Stephen B Knisley |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical optics Volume: 11 ISSN: 1083-3668 ISO Abbreviation: J Biomed Opt Publication Date: 2006 Mar-Apr |
Date Detail:
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Created Date: 2006-05-05 Completed Date: 2006-07-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 024019 Citation Subset: IM |
Affiliation:
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The University of North Carolina at Chapel Hill, Department of Biomedical Engineering, CB# 7575, 152 MacNider Hall, Chapel Hill, North Carolina 27599-7575, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Surface Potential Mapping / methods* Cells, Cultured Computer Simulation Heart Conduction System / physiology* Image Interpretation, Computer-Assisted / methods* Lighting / methods Microscopy, Fluorescence / methods* Models, Cardiovascular* Rabbits Spectrometry, Fluorescence / methods* |
| Grant Support | |
ID/Acronym/Agency:
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HL52003/HL/NHLBI NIH HHS; HL67728/HL/NHLBI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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