| Use of a laboratory exercise on molar absorptivity to help students understand the authority of the primary literature. | |
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MedLine Citation:
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PMID: 21591161 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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To promote understanding of the authority of the primary literature in students taking our biochemistry laboratory courses, a biochemistry laboratory exercise on the determination of an acceptable molar absorptivity value of 2-nitrophenol (2-NP) was developed. This made the laboratory course much more relevant by linking to a thematic thread, β-galactosidase, that scaffolds concepts in one exercise with those in later exercises. The substrate for the continuous assay of β-galactosidase is the chromogenic 2-nitrophenyl-β-D-galactopyranoside that produces 2-NP. In an early laboratory exercise, students determine the wavelength of maximum absorption for the protonated and deprotonated form of 2-NP at various pH values and then determine the molar absorptivity of 2-NP. Students were encouraged to discuss apparent discrepancies not only in their own determinations of molar absorptivity values for 2-NP, but also in the published molar absorptivity values for 2-NP (2,150-21,300 M(-1) cm(-1) ) at almost the same pH and at 420 nm. Finally, the students were led to a publication that serves as an authentic source for molar absorptivity of 2-NP. |
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Authors:
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Madhavan Soundararajan; Cheryl P Bailey; John Markwell |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology Volume: 36 ISSN: 1470-8175 ISO Abbreviation: Biochem Mol Biol Educ Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2011-05-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100970605 Medline TA: Biochem Mol Biol Educ Country: United States |
Other Details:
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Languages: eng Pagination: 61-4 Citation Subset: - |
Copyright Information:
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Copyright © 2008 International Union of Biochemistry and Molecular Biology, Inc. |
Affiliation:
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Center for Biological Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0664. msoundar@unlnotes.unl.edu. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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