Document Detail


Use of isoenzyme and PCR markers for analyzing human chromosomes in human-rodent hybrid cells
MedLine Citation:
PMID:  8316248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The possibility of using isozyme and PCR markers for estimation of preservation of human chromosomes in human.rodent somatic cell hybrids is considered. Methods of electrophoretic separation of 33 isozymes and 11 PCR markers for 22 human autosomes and X chromosome are described. Using these isozymes and PCR primers as markers of known regions and arms of human chromosomes, one can avoid errors in typization of chromosomes with complicated rearrangements which simulate similar patterns of G-banding. The method proposed in the paper not only facilitates considerably the analysis of hybrid clones, but is also an easy and reliable tool in selection of somatic cell hybrid clones for further investigation.
Authors:
T B Nestorova; E P Kopantsev; A M Borodin; S M Zakiian
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Molekuliarnaia biologiia     Volume:  27     ISSN:  0026-8984     ISO Abbreviation:  Mol. Biol. (Mosk.)     Publication Date:    1993 May-Jun
Date Detail:
Created Date:  1993-07-27     Completed Date:  1993-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0105454     Medline TA:  Mol Biol (Mosk)     Country:  RUSSIA    
Other Details:
Languages:  rus     Pagination:  685-707     Citation Subset:  IM    
Vernacular Title:
Ispol'zovanie izofermentnykh i PCR-markerov dlia analiza khromosom cheloveka v gibridnykh kletkakh chelovek-gryzun.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Chromosome Banding
Chromosomes, Human, Pair 22*
Cricetinae
Cricetulus
DNA, Single-Stranded
Electrophoresis, Polyacrylamide Gel
Genetic Markers*
Humans
Hybrid Cells*
Isoenzymes*
Mice
Molecular Sequence Data
Polymerase Chain Reaction
Rodentia
X Chromosome*
Chemical
Reg. No./Substance:
0/DNA, Single-Stranded; 0/Genetic Markers; 0/Isoenzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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