| Use of insulin-like growth factor-I during embryo culture and treatment of recipients with gonadotropin-releasing hormone to increase pregnancy rates following the transfer of in vitro-produced embryos to heat-stressed, lactating cows. | |
| | |
MedLine Citation:
|
PMID: 12817508 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An experiment was conducted to determine whether pregnancy rates following the transfer of in vitro-produced embryos to heat-stressed cows could be improved by 1) culturing embryos in the presence of IGF-I and 2) treating recipients with GnRH. Lactating Holstein cows (n = 260) were synchronized using a timed ovulation protocol. Embryos were produced in vitro and cultured with or without 100 ng/mL of IGF-I. On d 7 after anticipated ovulation (d 0), a single embryo was transferred to all recipients with a palpable corpus luteum (n = 210). A subset of recipients (n = 164) was injected with either GnRH or placebo on d 11. Plasma progesterone concentrations on d 0 and 7 were used to determine the synchrony of recipients. Pregnancy was diagnosed at d 53 and 81 by rectal palpation. Among all recipients, transfer of IGF-I-treated embryos increased pregnancy rate at d 53 (P < 0.05) and tended to increase pregnancy rate at d 81 (P < 0.06). Calving rate also tended to be higher for recipients that received IGF-I-treated embryos (P < 0.07). Among the subset of synchronized recipients (n = 190), pregnancy rate at d 53 and d 81 and calving rate were higher (P < 0.05) for IGF-I-treated embryos. The GnRH tended to increase pregnancy rate at d 53 for all recipients (P < 0.08) and the subset of synchronized recipients (P < 0.10). There were no effects of GnRH (P > 0.10) for pregnancy rate at d 81 and calving rate. The overall proportion of male calves was 64.3%. There was no effect (P > 0.10) of embryo treatment or GnRH on the birth weight or sex ratio of calves. Results of this experiment indicate that treatment of embryos with IGF-I can improve pregnancy and calving rates following transfer of in vitro-produced embryos. Further research is necessary to determine whether the treatment of recipients with GnRH is a practical approach to increase pregnancy rates following in vitro embryo transfer. |
| | |
Authors:
|
J Block; M Drost; R L Monson; J J Rutledge; R M Rivera; F F Paula-Lopes; O M Ocon; C E Krininger; J Liu; P J Hansen |
Related Documents
:
|
15567878 - Would legislation imposing single embryo transfer be a feasible way to reduce the rate ... 1391648 - Gamete and zygote intrafallopian transfers and related techniques. 10469708 - Characterization of a top quality embryo, a step towards single-embryo transfer. 1582938 - Bioeconomic evaluation of embryo transfer in beef production systems: ii. economic eval... 8780938 - Three siblings with walker-warburg syndrome. 76068 - Maternal hyperthermia as a possible cause of anencephaly. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Journal of animal science Volume: 81 ISSN: 0021-8812 ISO Abbreviation: J. Anim. Sci. Publication Date: 2003 Jun |
Date Detail:
|
Created Date: 2003-06-23 Completed Date: 2003-12-08 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 1590-602 Citation Subset: IM |
Affiliation:
|
Department of Animal Sciences, University of Florida, Gainesville 32611, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Birth Weight / drug effects Cattle / embryology, physiology* Culture Media Embryo Transfer / veterinary* Female Gonadotropin-Releasing Hormone / administration & dosage* Hot Temperature* Insemination, Artificial / veterinary Insulin-Like Growth Factor I / administration & dosage* Lactation Male Pregnancy Pregnancy Rate* Progesterone / blood Sex Ratio Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 33515-09-2/Gonadotropin-Releasing Hormone; 57-83-0/Progesterone; 67763-96-6/Insulin-Like Growth Factor I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of dietary energy source on serum concentration of insulin-like growth factor-I growth ho...
Next Document: Effect of soil type and fertilization level on mineral concentration of pasture: potential relations...