Document Detail


Use of immobilized L-asparaginase in acrylic microparticles in an extracorporeal hollow-fiber dialyzer.
MedLine Citation:
PMID:  6834269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
L-asparaginase was immobilized under aseptic conditions in spherical microparticles of polyacrylamide. To avoid direct contact between blood and enzyme, we have applied the immobilized L-asparaginase in microparticles on the outer surface of the capillary fibers of a hemofilter. The hemofilters were very efficient in the transformation of L-asparagine to L-aspartic acid, both in vitro and in vivo. L-Asparagine in buffer (50 microM in 5 liters) was converted to L-aspartic acid within 60 min after circulation through a hemofilter containing 2000 I.U. of L-asparaginase. Circulating L-asparagine in healthy sheep (about 40-50 microM was reduced to low levels after 2 to 3 hr of perfusion with a unit containing 2000 I.U. of L-asparaginase. The reduction persisted for 3 to 4 hr after terminated treatment. Repeated, extracorporeal treatments in sheep showed that the L-asparagine decrease induced an increased resynthesis of L-asparagine, probably due to the action of the L-asparagine synthetase.
Authors:
P Edman; U Nylén; I Sjöholm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  225     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-05-27     Completed Date:  1983-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  164-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins
Animals
Asparaginase / administration & dosage,  metabolism,  pharmacology*
Asparagine / blood
Aspartic Acid / blood
Blood
Microspheres
Perfusion
Renal Dialysis / methods*
Sheep
Ultrafiltration
Chemical
Reg. No./Substance:
0/Acrylic Resins; 56-84-8/Aspartic Acid; 7006-34-0/Asparagine; 9003-05-8/polyacrylamide; EC 3.5.1.1/Asparaginase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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