| Use of high performance liquid chromatography in defining the abnormalities in the free amino acid patterns in the cerebrospinal fluid of patients with aseptic meningitis. | |
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MedLine Citation:
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PMID: 1683804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Free amino acids were quantitatively determined in cerebrospinal fluid (CSF) and plasma samples from patients with aseptic meningitis by a newly developed high performance liquid chromatographic (HPLC) method. The method of analysis was based on precolumn derivatization of orthophthaladehyde in the presence of 2-mercaptoethanol and detection was made at Eex = 340 nm and Eem = 450 nm. The method was sensitive and the limit for detection was less than 1 pmol for most of the amino acids. It took 45 min to separate 26 amino acids with highly reproducible results, giving a coefficient of variance for retention times and integrated areas less than 0.4% and 2%, respectively, after five replicate runs. The results accumulated in 10 patients were compared statistically with 11 age-matched healthy controls. Among the amino acids almost all the neurotransmitter candidates, such as aspartic acid, glutamic acid, glutamine, glycine, tyrosine, phenylalanine and gamma-aminobutyric acid (GABA), were significantly increased in the patients' CSF, whereas arginine and threonine were low. No change was observed in plasma amino acids in patients as compared to healthy controls. The higher levels of most of the neurotransmitters, especially GABA, aspartic acid and glutamic acid, could be used diagnostically in assessing the progression and remission in aseptic meningitis. |
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Authors:
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I Halawa; S Baig; G A Qureshi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 5 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1992-01-14 Completed Date: 1992-01-14 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 216-20 Citation Subset: IM |
Affiliation:
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Department of Neurology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Amino Acids / blood, cerebrospinal fluid* Aspartic Acid / blood, cerebrospinal fluid Chromatography, High Pressure Liquid / methods* Female Glutamates / blood, cerebrospinal fluid Glutamic Acid Glycine / blood, cerebrospinal fluid Humans Male Meningitis, Aseptic / blood, cerebrospinal fluid* Middle Aged Phenylalanine / blood, cerebrospinal fluid Tyrosine / blood, cerebrospinal fluid gamma-Aminobutyric Acid / blood, cerebrospinal fluid |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Glutamates; 55520-40-6/Tyrosine; 56-12-2/gamma-Aminobutyric Acid; 56-40-6/Glycine; 56-84-8/Aspartic Acid; 56-86-0/Glutamic Acid; 63-91-2/Phenylalanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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