| Use of granulocyte colony-stimulating factor for fluorine-18-fluorodeoxyglucose labeling in human leukocytes. | |
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MedLine Citation:
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PMID: 21178645 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: Insufficient labeling efficiency and poor retention of radioactivity are the considerable shortcomings of fluorine-18-fluorodeoxyglucose (F-FDG) labeling in human leukocytes. This study was conducted toevaluate the feasibility of using granulocyte colony-stimulating factor (G-CSF) to improve F-FDG labeling in human leukocytes. METHODS: Leukocyte separation was performed using methods reported earlier. Separated leukocytes were preincubated with G-CSF or insulin at 37°C for 1 h. Afterpreincubation, labeling was performed with F-FDG (37-74 MBq) at 37°C for 30 min. Retained radioactivity was assessed at 1-4 h after labeling by the same method described in earlier reports. The viability of labeled leukocytes was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. RESULTS: Labeling efficiency of leukocytes preincubated withG-CSF, G-CSF with insulin, insulin, and control leukocytes was 52.1±8.9%, 49.9±10.5%, 40.3±7.7%, and 40.3±6.0%, respectively. G-CSF significantly increased the labeling efficiency compared with insulin (P=0.005) and control (P=0.004). In leukocytes preincubated with G-CSF, 77.0±1.2%, and 59.0±1.8% of radioactivity was retained at 1 and 3 h after labeling. There was no significant difference in retained radioactivity compared with that of leukocytes with different treatment at all time points. Furthermore, no significant difference in viabilities among leukocytes with different treatments was observed. CONCLUSION: Use of G-CSF significantly improved F-FDG labeling efficiency without a significant effect on cell viability and retention of radioactivity. |
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Authors:
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Seung Hwan Moon; Ho-Young Lee; Jae Seon Eo; Seog Gyun Kim; Hye-Kyung Shim; Hyun Woo Kwon; Chulhan Kim; Yong-Il Kim; Dong Soo Lee; June-Key Chung; Myung Chul Lee |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nuclear medicine communications Volume: 32 ISSN: 1473-5628 ISO Abbreviation: Nucl Med Commun Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-01-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8201017 Medline TA: Nucl Med Commun Country: England |
Other Details:
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Languages: eng Pagination: 186-91 Citation Subset: IM |
Affiliation:
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aDepartment of Nuclear Medicine, Seoul National University College of Medicine bSeoul Metropolitan Government, Seoul National University Boramae Medical Center, Seoul, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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