Document Detail


Use of glycerol as an optical clearing agent for enhancing photonic transference and detection of Salmonella typhimurium through porcine skin.
MedLine Citation:
PMID:  17092176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to evaluate glycerol (GLY) and GLY + dimethyl sulfoxide (DMSO) to increase photonic detection of transformed Salmonella typhimurium (S. typh-lux) through porcine skin. Skin was placed on 96-well plates containing S. typh-lux, imaged (5 min) using a CCD camera, and then completely immersed in PBS, GLY, DMSO, GLY+DMSO in a dose- and time-dependent manner and re-imaged (5 min). The percent of photonic emissions detected (treated or untreated skin relative to no skin controls) was used for analysis. Treatment for 4 h with 50% GLY-PBS and 50:30:20% GLY:DMSO:PBS increased photonic detection compared to untreated skin, 100% PBS, or 30:70% DMSO:PBS. Treatment with 50% GLY in the presence of 20 and 40% DMSO (v/v with PBS) increased photonic detection compared to 50% GLY alone and in the presence of 10% DMSO: 50% GLY (v/v with PBS). Data indicate that GLY and GLY+DMSO are effective optical clearing agents on porcine skin (2-3 mm thick) when treated for 4 h to increase detection of emitted photons. Clearing agents such as GLY have the potential to minimize effects of porcine skin tissue as one of the photon transmittance barriers (i.e., skin, fat, muscle, and visceral tissues) in vivo.
Authors:
Keesla Moulton; Frank Lovell; Ellen Williams; Peter Ryan; Donald C Lay; Duco Jansen; Scott Willard
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  11     ISSN:  1083-3668     ISO Abbreviation:  J Biomed Opt     Publication Date:    2006 Sep-Oct
Date Detail:
Created Date:  2006-11-09     Completed Date:  2007-01-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  054027     Citation Subset:  IM    
Affiliation:
Department of Animal and Dairy Science, Mississippi State University, Box 9815 Mississippi State, Mississippi 39762, USA.
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Animals
Colony Count, Microbial / methods*
Glycerol / administration & dosage*
Salmonella typhimurium / isolation & purification*
Skin / drug effects*,  microbiology*
Skin Physiological Phenomena / drug effects*
Spectrum Analysis / methods*
Swine
Chemical
Reg. No./Substance:
56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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