Document Detail


Use of the glucosyltransferase UGT71B6 to disturb abscisic acid homeostasis in Arabidopsis thaliana.
MedLine Citation:
PMID:  16623908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A glucosyltransferase (GT) of Arabidopsis, UGT71B6, recognizing the naturally occurring enantiomer of abscisic acid (ABA) in vitro, has been used to disturb ABA homeostasis in planta. Transgenic plants constitutively overexpressing UGT71B6 (71B6-OE) have been analysed for changes in ABA and the related ABA metabolites abscisic acid glucose ester (ABA-GE), phaseic acid (PA), dihydrophaseic acid (DPA), 7'-hydroxyABA and neo-phaseic acid. Overexpression of the GT led to massive accumulation of ABA-GE and reduced levels of the oxidative metabolites PA and DPA, but had marginal effect on levels of free ABA. The control of ABA homeostasis, as reflected in levels of the different metabolites, differed in the 71B6-OEs whether the plants were grown under standard conditions or subjected to wilt stress. The impact of increased glucosylation of ABA on ABA-related phenotypes has also been assessed. Increased glucosylation of ABA led to phenotypic changes in post-germinative growth. The use of two structural analogues of ABA, known to have biological activity but to differ in their capacity to act as substrates for 71B6 in vitro, confirmed that the phenotypic changes arose specifically from the increased glucosylation caused by overexpression of 71B6. The phenotype and profile of ABA and related metabolites in a knockout line of 71B6, relative to wild type, has been assessed during Arabidopsis development and following stress treatments. The lack of major changes in these parameters is discussed in the context of functional redundancy of the multigene family of GTs in Arabidopsis.
Authors:
David M Priest; Stephen J Ambrose; Fabián E Vaistij; Luisa Elias; Gillian S Higgins; Andrew R S Ross; Suzanne R Abrams; Dianna J Bowles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  46     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-20     Completed Date:  2006-07-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  492-502     Citation Subset:  IM    
Affiliation:
CNAP, Department of Biology (Area 8), University of York, York YO10 5YW, UK.
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / analogs & derivatives,  metabolism*
Arabidopsis / enzymology*,  genetics,  growth & development
Arabidopsis Proteins / genetics,  physiology*
Esters
Glycosyltransferases / genetics,  physiology*
Homeostasis
Multigene Family / physiology
Phenotype
Plant Leaves / genetics,  growth & development,  metabolism
Plants, Genetically Modified / enzymology
Water / metabolism
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Esters; 21293-29-8/Abscisic Acid; 7732-18-5/Water; EC 2.4.-/Glycosyltransferases; EC 2.4.-/UGT71B6 protein, Arabidopsis

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