| Use of gaseous 13NH3 administered to intact leaves of Nicotiana tabacum to study changes in nitrogen utilization during defence induction. | |
| | |
MedLine Citation:
|
PMID: 20716065 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Nitrogen-13 (t(1/2) 9.97 m), a radioactive isotope of nitrogen, offers unique opportunities to explore plant nitrogen utilization over short time periods. Here we describe a method for administering (13)N as gaseous (13)NH(3) to intact leaves of Nicotiana tabacum L. (cv Samsun), and measuring the labelled amino acids using radio high-performance liquid chromatography (HPLC) on tissue extract. We used this method to study the effects of defence induction on plant nitrogen utilization by applying treatments of methyl jasmonate (MeJA), a potent defence elicitor. MeJA caused a significant increase relative to controls in key [(13)N]amino acids, including serine, glycine and alanine by 4 h post-treatment, yet had no effect on (13)NH(3) incorporation, a process that is primarily under the control of the glutamine synthatase/glutamate synthase pathway (GS/GOGAT) in cellular photorespiration. We suggest that the reconfiguration of nitrogen metabolism may reflect induction of non-photorespiratory sources of nitrogen to better serve the plant's defences. |
| | |
Authors:
|
Nils Hanik; Sara Gómez; Michael Schueller; Colin M Orians; Richard A Ferrieri |
Related Documents
:
|
16911515 - A new physiological role for pdr12p in saccharomyces cerevisiae: export of aromatic and... 16831715 - Food energy values of artificial feeds for man. 14968425 - Four permeases import proline and the toxic proline analogue azetidine-2-carboxylate in... 12597405 - Effects of urea infusion and ruminal degradable protein concentration on microbial grow... 8173925 - Biliary bile acid profiles in patients with familial adenomatous polyposis before and a... 11872115 - Acid stress in the food pathogen bacillus cereus. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-09-10 |
Journal Detail:
|
Title: Plant, cell & environment Volume: 33 ISSN: 1365-3040 ISO Abbreviation: Plant Cell Environ. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9309004 Medline TA: Plant Cell Environ Country: United States |
Other Details:
|
Languages: eng Pagination: 2173-9 Citation Subset: IM |
Copyright Information:
|
© 2010 Blackwell Publishing Ltd. |
Affiliation:
|
Fachbereich Chemie, Johannes Gutenberg Universität, Mainz, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A variety of risk topics and risk processes within different settings.
Next Document: Phloem-derived ?-aminobutyric acid (GABA) is involved in upregulating nodule N2 fixation efficiency ...