| Use of gamma-spectrometry for simultaneous determination of 210Pb, 73As, 109Cd, 203Hg and 59Fe distribution and excretion in rats at low doses. | |
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MedLine Citation:
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PMID: 10996664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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gamma-Spectrometry permits the identification and quantification of different gamma-isotopes in the same aliquot. To estimate the sensitivity and discriminative power of a comparably small and inexpensive 8% germanium detector, we determined the detection limits for simultaneously applied 210Pb, 73As, 109Cd, 203Hg and 59Fe. The concentration of Fe and of each of the four potential environmental contaminants was determined in aliquots from all organs and tissues 10 days after simultaneous i.v. administration (2 micromol/kg body weight) to adult and growing iron-deficient and iron-adequate rats. Relating these values to the total size of each organ permitted to derive a whole body distribution pattern for all five isotopes in each individual animal. Cumulative renal and faecal excretion values were determined during the 10 day distribution period to calculate the half-lives for both excretory pathways for all five isotopes simultaneously. Distribution and excretion values corresponded well to literature data. Extrapolation of the results showed that the detector would be sensitive enough to discriminate and quantify the five metals at human dietary exposure levels. The results recommend to use gamma-spectrometry to investigate kinetic aspects of interactions between toxic and essential trace metals, because the method reduces the number of required animals drastically. |
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Authors:
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G Hunder; J Javdani; B Elsenhans; K Schümann |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Toxicology Volume: 150 ISSN: 0300-483X ISO Abbreviation: Toxicology Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-10-26 Completed Date: 2000-10-26 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0361055 Medline TA: Toxicology Country: IRELAND |
Other Details:
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Languages: eng Pagination: 69-82 Citation Subset: IM |
Affiliation:
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Walther-Straub-lnstitut für Pharmakologie und Toxikologie der Ludwig-Maximilians-Universität, Nussbaumstr. 26, München, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arsenic / pharmacokinetics* Body Weight Cadmium / pharmacokinetics* Iron / pharmacokinetics* Lead / pharmacokinetics* Male Mercury / pharmacokinetics* Organ Size Rats Rats, Sprague-Dawley Sensitivity and Specificity Spectrometry, Gamma Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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7439-89-6/Iron; 7439-92-1/Lead; 7439-97-6/Mercury; 7440-38-2/Arsenic; 7440-43-9/Cadmium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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