Document Detail


Use of gamma-inulin/liposomes/Vitamin E adjuvant combination in contraceptive vaccines.
MedLine Citation:
PMID:  12711164     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The adjuvanticity of two gamma inulin/liposomes/Vitamin E combinations was evaluated in the mouse, in contraceptive vaccines with sperm protein extracts or a synthetic HE2 peptide ("Human Epididymis gene product"; residues 15-28) as antigen. The HE2 peptide was not conjugated to a protein carrier, to ensure that the antibodies elicited were specific against the HE2 peptide. The adjuvant combinations were designed to increase adjuvanticity, as their components have complementary mechanisms, and their performance was compared to Freund's adjuvant. Antibody production against native sperm structures was determined in sera by ELISA immunoassay and immunohistology. Toxicity of adjuvants was determined by histopathological study and treated mice were monitored for signs of pain or distress. Our results show that the gamma inulin (1-2 microm particle size)/liposomes/Vitamin E combination, with sperm protein extracts, is better than Freund's adjuvant because it elicits good antibody titres without any toxicity. When the synthetic HE2 peptide is used as antigen, the gamma inulin (1-2 microm particle size)/liposomes/Vitamin E combination is less effective than Freund's adjuvant; nevertheless, the anti-HE2 antibodies elicited are highly specific and recognize native structures in sperm.
Authors:
P Fuentes; P D Cooper; R Barnadas; M Sabés; C Osterhoff; P Martínez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  257     ISSN:  0378-5173     ISO Abbreviation:  Int J Pharm     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-24     Completed Date:  2003-05-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  85-95     Citation Subset:  IM    
Affiliation:
Immunology Unit, Instituto de Biomedicina y Biotecnología, Universidad Autónoma de Barcelona, Barcelona 08193, Bellaterra, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adjuvants, Immunologic / administration & dosage*
Animals
Antibody Formation
Antigens, Surface / administration & dosage*,  immunology*
Glycopeptides / administration & dosage*,  immunology*
Immunization
Inulin / administration & dosage*
Liposomes / administration & dosage
Mice
Microscopy, Fluorescence
Recombinant Proteins / administration & dosage*,  immunology*
Vaccines, Contraceptive*
Vitamin E / administration & dosage*
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Antigens, Surface; 0/Glycopeptides; 0/Liposomes; 0/Recombinant Proteins; 0/SPAG11A protein, human; 0/Vaccines, Contraceptive; 1406-18-4/Vitamin E; 9005-80-5/Inulin

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