Document Detail

Use of fluorescent microspheres as a tool to investigate bacterial interactions with growing plants.
MedLine Citation:
PMID:  15830687     Owner:  NLM     Status:  MEDLINE    
Foodborne pathogens may exist as endophytes of growing plants. The internalization of Escherichia coli O157:H7 or other foodborne pathogens in growing lettuce plants may be independent of microbial factors. Mature lettuce plants were surface irrigated with E. coli O157:H7 or with FluoSpheres (fluorescent microspheres) and harvested 1, 3, and 5 days post-exposure. FluoSpheres were utilized as a bacterial surrogate. Microscopic examination of root, stem, and leaf tissue sections revealed that FluoSpheres were internalized into growing plants. Laser scanning confocal microscopy revealed that FluoSpheres were present within the root tissue and leaf stem tissue. The presence of FluoSpheres in internal portions of stem and leaf tissue suggests transport of the spheres from the root upward into the edible tissue. The level of uptake of FluoSpheres and E. coli O157:H7 was quantified using filtration. Numbers of FluoSpheres and E. coli O157:H7 cells in plant tissue were similar. The entry of E. coli O157:H7 into lettuce plants may be a passive event because the concentration of FluoSpheres was similar to that of the pathogen.
Ethan B Solomon; Karl R Matthews
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food protection     Volume:  68     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-15     Completed Date:  2005-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  870-3     Citation Subset:  IM    
Department of Food Science, Rutgers University, New Brunswick, New Jersey 08901, USA.
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MeSH Terms
Bacterial Adhesion
Colony Count, Microbial
Escherichia coli O157 / growth & development*,  physiology
Food Microbiology*
Lettuce / microbiology*
Microscopy, Confocal
Plant Leaves / microbiology,  ultrastructure
Plant Roots / microbiology,  ultrastructure

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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