Document Detail

Use of fluorescence in situ hybridization to detect loss of chromosome 10 in astrocytomas.
MedLine Citation:
PMID:  7616278     Owner:  NLM     Status:  MEDLINE    
Models describing progression in the genetic derangement of glial tumors have shown loss of chromosome 10 to occur most frequently in high-grade lesions, suggesting that identification of this loss may be prognostically significant. Fluorescence in situ hybridization (FISH) analysis may be a valuable adjunct to histological grading if it can accurately detect this loss. In this paper, the authors correlate results obtained from FISH, cytogenetic, molecular genetic, and flow cytometric analyses of a series of 39 brain specimens, including seven normal, two gliotic, and 30 neoplastic (one Grade II, one Grade III, and 28 Grade IV astrocytoma) specimens. Contiguous section of freshly resected surgical tissue were submitted for tissue culturing (karyotype) and touch preparation (FISH), snap-frozen (molecular genetic), or paraffin-embedded (histology and flow cytometry). Centromere-specific probes for chromosomes 10 and 12 were used for FISH analysis, and 19 restriction fragment length polymorphisms (two p-arm and 17 q-arm) and four microsatellite sequence polymorphisms (three p-arm and one q-arm) were used for molecular genetic analysis of chromosome 10. Findings showed FISH and loss of heterozygosity (LOH) analyses to be concordant in 33 of 38 specimens (sensitivity 94%, specificity 81%), with one specimen indeterminate on LOH analysis. Both FISH and LOH analyses were more sensitive at detecting chromosome 10 loss than conventional cytogenetic (karyotype) analysis. The authors conclude that FISH is a sensitive test for detecting chromosome 10 loss and ploidy in astrocytic tumors.
S J Dalrymple; J F Herath; S R Ritland; C A Moertel; R B Jenkins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurosurgery     Volume:  83     ISSN:  0022-3085     ISO Abbreviation:  J. Neurosurg.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-08-24     Completed Date:  1995-08-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0253357     Medline TA:  J Neurosurg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  316-23     Citation Subset:  AIM; IM    
Department of Neurologic Surgery, Mayo Medical Center, Rochester, Minnesota, USA.
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MeSH Terms
Aged, 80 and over
Astrocytoma / genetics*
Brain / metabolism
Brain Neoplasms / genetics*
Chromosome Deletion
Chromosomes, Human, Pair 10 / genetics*
Chromosomes, Human, Pair 12 / genetics
Chromosomes, Human, Pair 17 / genetics
Glioma / genetics
In Situ Hybridization*
Middle Aged
Polymorphism, Genetic / genetics
Polymorphism, Restriction Fragment Length
Sensitivity and Specificity
Grant Support

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