| Use of flow cytometry with fluorescent antibodies in real-time monitoring of simultaneously inoculated alcoholic-malolactic fermentation of Chardonnay. | |
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MedLine Citation:
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PMID: 17944859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To monitor in real-time the changes in microbial populations and chemistry of grape juice simultaneously inoculated with Saccharomyces cerevisiae and Oenococcus oeni. METHODS AND RESULTS: Viable populations of S. cerevisiae and O. oeni in Chardonnay fermentations were identified and quantified using fluorescent dyes and fluorescently labelled antibodies in a flow cytometric assay. Fermentation chemistry was monitored using Fourier transform infrared (FTIR) spectroscopy, except for malic acid which was measured enzymatically. Malic acid utilization by O. oeni was greatest in the presence of the yeast Cepage. Growth of O. oeni was substantially slower in the presence of the yeast VL1. The three yeasts had similar fermentation rates in the presence and absence of O. oeni. CONCLUSIONS: Viable and nonviable yeast and bacterial populations can be rapidly discriminated in simultaneous malolactic-alcoholic wine fermentations using antibodies, fluorescent dyes and flow cytometry. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study using fluorescently labelled antibodies to discriminate and monitor yeast and bacterial populations in wine fermentations and offers a new approach to investigating microbial interactions in wine fermentations. |
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Authors:
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S B Rodriguez; R J Thornton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-16 |
Journal Detail:
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Title: Letters in applied microbiology Volume: 46 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-01-09 Completed Date: 2008-04-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 38-42 Citation Subset: IM |
Affiliation:
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Department of Viticulture and Enology, California State University, Fresno, California 93740, USA. susanr@csufresno.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Colony Count, Microbial Ethanol / metabolism* Fermentation* Flow Cytometry / instrumentation Fluorescent Antibody Technique / methods* Leuconostoc / growth & development, isolation & purification, metabolism Malates / metabolism* Microbial Viability Saccharomyces cerevisiae / growth & development, isolation & purification, metabolism Spectroscopy, Fourier Transform Infrared Wine / analysis, microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Malates; 64-17-5/Ethanol; 6915-15-7/malic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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