Document Detail


Use of flow-cytometric analysis to optimize cell banking strategies for production of biopharmaceuticals from mammalian cells.
MedLine Citation:
PMID:  16043250     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Production of biopharmaceuticals from mammalian cells requires generation of master, working and post-production cell banks of high quality under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to establishing production processes that are completely devoid of serum or even any animal components and to ensuring robust thaw performance for reliable production. Here, we describe a novel strategy employing flow-cytometric (FC) analysis of Annexin V-stained cells for high-throughput characterization of cell banks. Our data show that this method enables predictive evaluation of a cryopreservation strategy as early as 6h after thawing of cells. Furthermore, a broad study is presented characterizing various factors that may influence the quality of serum-free production cell banks from NSO and CHO cell lines. These results demonstrate how FC-based analysis can be used for development of future state-of-the-art cryopreservation strategies.
Authors:
Jürgen Fieder; Nina Wagner; Stefanos Grammatikos; Helmut Hoffmann; Hitto Kaufmann; Ralf T Otto
Publication Detail:
Type:  Journal Article     Date:  2005-07-25
Journal Detail:
Title:  Journal of biotechnology     Volume:  120     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-05     Completed Date:  2005-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  111-20     Citation Subset:  IM    
Affiliation:
Boehringer Ingelheim Pharma GmbH & Co. KG, BP Process Science, 88397 Biberach an der Riss, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopharmaceutics / methods*
CHO Cells
Cell Culture Techniques / methods*
Cell Line, Tumor
Cricetinae
Cryopreservation / methods*
Flow Cytometry / methods*
Mammals
Mice
Multiple Myeloma / metabolism*
Pharmaceutical Preparations / metabolism*
Protein Engineering / methods*
Chemical
Reg. No./Substance:
0/Pharmaceutical Preparations

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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