| Use of the enzyme-linked immunoadsorbent assay to monitor the purification of glycosphingolipid antigens by high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 3479028 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An enzyme-linked immunoadsorbent assay (ELISA) technique has been applied to the analysis of glycosphingolipid fractions separated by high-performance liquid chromatography. Nanogram amounts of selected fractions were placed in microtiter wells and analyzed for glycosphingolipids carrying carbohydrate epitopes recognized by monoclonal antibodies using an avidin-biotin enzyme system (ABC reagents). A large number of fractions (more than 100) can be conveniently evaluated for the presence of glycosphingolipids recognized by one or more monoclonal antibodies in a single analysis. This method is a rapid and sensitive procedure for monitoring the purification of glycosphingolipid antigens and can be used in conjunction with immunostaining of glycosphingolipids separated by thin-layer chromatography. |
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Authors:
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J Buehler; U Galili; B A Macher |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 164 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1987 Aug |
Date Detail:
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Created Date: 1987-12-04 Completed Date: 1987-12-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 521-5 Citation Subset: IM |
Affiliation:
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Cancer Research Institute, Department of Pharmaceutical Chemistry, University of California, San Francisco 94143. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal Antigens / isolation & purification* Chromatography, High Pressure Liquid / methods* Enzyme-Linked Immunosorbent Assay* Erythrocytes / analysis, immunology Glycosphingolipids / blood, immunology, isolation & purification* Humans Leukemia, Myeloid / blood, immunology Rabbits |
| Grant Support | |
ID/Acronym/Agency:
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AG-06299/AG/NIA NIH HHS; CA-32826/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens; 0/Glycosphingolipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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