Document Detail


Use of an encapsulated fluorescent probe to measure intracellular PO2.
MedLine Citation:
PMID:  7251688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objectives of this investigation were to produce a reliable, sensitive probe to measure intracellular PO2 with a high degree of resolution and to apply this technique to biological systems. A fluorescent molecule, pyrene dissolved in paraffin oil, was encapsulated in polyacrylamide to form a probe of nanometer dimensions. The quantitative and microscopic oxygen values were determined by analyzing the quenching of the fluorescence of the probe by oxygen, as displayed on a television monitor by a silicon-intensified-target camera. The nanocapsules had a sensitivity of approximately 1 mm PO2, a spatial resolution of 0.5 micrometer, and a temporal resolution of milliseconds. Calibrated nanocapsules within nonrespiring Amoeba proteus responded to ambient partial pressures of oxygen. At two different ambient partial pressures, nanocapsules engulfed by respiring amoebas indicated an intracellular PO2 28 mm Hg less than extracellular PO2. The capsules retained their sensitivity to oxygen for at least 8 months.
Authors:
G T Podgorski; I S Longmuir; J A Knopp; D M Benson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  107     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1981-09-22     Completed Date:  1981-09-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  329-34     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amoeba / physiology
Animals
Fluorescent Dyes*
Intracellular Membranes / physiology*
Oils / pharmacology
Oxygen*
Paraffin / pharmacology
Partial Pressure
Pyrenes / pharmacology
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Oils; 0/Pyrenes; 129-00-0/pyrene; 7782-44-7/Oxygen; 8002-74-2/Paraffin; 8012-95-1/paraffin oils

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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